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Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

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Related Experiment Video

Updated: Apr 3, 2026

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

A Rapid and Visual Soybean Hairy Root Transformation Protocol Using the RUBY Reporter.

Zhirui Zhang¹, Qiushi Wang^1,2, Yu Geng¹

¹Key Laboratory of Soybean Molecular Design Breeding, State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, China.

|April 2, 2026

Summary

This summary is machine-generated.

This study introduces a simplified soybean hairy root transformation method using the RUBY reporter, eliminating tissue culture for faster gene function analysis. Transgenic roots are visually identified by red color, streamlining experiments.

Keywords:

Agrobacterium rhizogenes Hairy root transformation RUBY visual reporter Soybean Tissue culture‐independent

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Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat Fagopyrum tataricum

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat Fagopyrum tataricum

Published on: March 11, 2020

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Last Updated: Apr 3, 2026

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

Author Spotlight: Streamlining Composite Plant Production via Agrobacterium rhizogenes-Mediated Hairy Root Transformation

Author Spotlight: Streamlining Composite Plant Production via Agrobacterium rhizogenes-Mediated Hairy Root Transformation

Published on: June 30, 2023

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat Fagopyrum tataricum

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat Fagopyrum tataricum

Published on: March 11, 2020

Area of Science:

Plant biotechnology
Molecular biology
Genetics

Background:

Agrobacterium rhizogenes-mediated hairy root transformation is crucial for rapid gene function analysis.
Existing methods often require tissue culture and complex selection, hindering efficiency.

Purpose of the Study:

To develop a streamlined, tissue culture-free soybean hairy root transformation protocol.
To utilize the RUBY visual reporter for direct identification of transgenic roots.

Main Methods:

Agrobacterium rhizogenes-mediated transformation of soybean.
Incorporation of the RUBY visual reporter system for phenotypic selection.
Elimination of traditional tissue culture and chemical selection steps.

Main Results:

Transgenic soybean hairy roots were generated without tissue culture.
RUBY-positive roots were directly identifiable via red pigmentation.
Effective gene expression was confirmed in RUBY-positive roots.
Identifiable transgenic roots were obtained within 20 days.

Conclusions:

The developed protocol simplifies soybean hairy root transformation, reducing complexity and equipment needs.
This method enables rapid functional analysis of genes, proteins, and root-microbe interactions.
Visual identification via RUBY reporter accelerates screening and experimental timelines.