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Related Concept Videos

Centrioles and Centrosomes01:13

Centrioles and Centrosomes

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Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or...
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Centrosome Duplication02:25

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The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
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Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
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The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
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Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
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Related Experiment Video

Updated: Apr 4, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
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Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

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Centriolar satellites regulate CEP350 mRNA localization and centrosome amplification.

Abraham Martinez1, Chad G Pearson1

  • 1Department of Cell and Developmental Biology, University of Colorado, Anschutz Medical Campus, Aurora, CO 80045.

Biorxiv : the Preprint Server for Biology
|April 3, 2026
PubMed
Summary
This summary is machine-generated.

Messenger RNAs (mRNAs) for CEP350 protein localize to centrosomes via CEP131 and Unkempt (UNK) during S phase. This pathway is crucial for centrosome amplification in triple-negative breast cancer, offering potential therapeutic targets.

Keywords:
RNA binding proteinRNA localizationcentriolar satellitecentriolecentrosomelocal translationmicrotubule

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Related Experiment Videos

Last Updated: Apr 4, 2026

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09:39

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Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
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Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

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Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • Messenger RNAs (mRNAs) are known to accumulate at centrosomes, but the mechanisms and functional importance of this localization are not fully understood.
  • Centrosomal localization of specific mRNAs, such as those encoding centrosomal proteins, occurs during interphase, yet the underlying processes remain unclear.

Purpose of the Study:

  • To investigate the regulation and function of the centrosome-localized mRNA, CEP350.
  • To elucidate the mechanisms governing CEP350 mRNA localization to centrosomes and its functional significance.

Main Methods:

  • Investigated mRNA localization using techniques to track CEP350 mRNA.
  • Utilized RNA binding protein (RBP) and protein interaction studies.
  • Examined microtubule (MT) dependence of mRNA localization.
  • Assessed the role of identified proteins in centriole duplication and centrosome amplification.

Main Results:

  • CEP350 mRNA localizes to centrosomes during S phase, dependent on CEP131 and Unkempt (UNK) in a microtubule-dependent manner.
  • CEP131 and UNK stabilize CEP350 mRNA, maintaining its steady-state levels and promoting normal CEP350 protein levels at centrosomes.
  • CEP350 is essential for PLK4-induced centriole overduplication and plays a role in centrosome amplification in triple-negative breast cancer cells.

Conclusions:

  • A novel centriolar satellite-RNA binding protein (RBP) pathway regulates CEP350 mRNA localization to centrosomes.
  • CEP131, UNK, and CEP350 are critical for centrosome amplification in triple-negative breast cancer, identifying them as potential therapeutic targets.