Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic biomimicry in skin-on-a-chip: multi-scale construction to translational dermatology, drug screening and cosmetic evaluation.

Lab on a chip·2026
Same author

A Multi-Domain Affinity Metal Organic Polymer-MXene Heterostructure for Superior Advanced Antibody Separation.

Analytical chemistry·2026
Same author

Enhancing Biotechnological Applications Using an Optimized Semiconductor Refrigeration Device.

ACS applied materials & interfaces·2025
Same author

ZnFe Layered Double Hydroxide Nanosheets Loaded with Cu Single-Atom Nanozymes with Multi-Enzyme-Like Catalytic Activities as an Effective Treatment for Bacterial Keratitis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Ultrasound-Guided High-Intensity Focused Ultrasound Combined With PD-1 Blockade in Patients With Liver Metastases From Lung Cancer: Protocol for a Single-Arm Phase 2 Trial.

JMIR research protocols·2024
Same author

Promoting CO<sub>2</sub> Electroreduction Over ion-exchange Resin-derived Ni-N-C Catalyst with Sulfur Doping.

Chemistry, an Asian journal·2024
Same journal

Modeling the Effects of Short-Range Randomness in Packed Sphere Beds.

Analytical chemistry·2026
Same journal

Mitochondrial Redox Cascade-Directed Covalent NIR Fluorogenic Imaging of Therapy-Induced Senescence Integrates Tumor and Host Responses.

Analytical chemistry·2026
Same journal

Proteomic Profiling of RHD-Related Mitral Annulus Calcification Enabled by Magnetic Carbon Nanomaterial-Supported Quasi-Immobilized Enzyme Digestion.

Analytical chemistry·2026
Same journal

Spatial-Photonic Encoding on a Single Fiber: Breaking the Bottleneck in Photoelectrochemical Biosensing for Precision Diagnostics.

Analytical chemistry·2026
Same journal

Spreadable Biosensing Pregel for Analyte Visualization in Peeled Plant Tissues.

Analytical chemistry·2026
Same journal

DARibo-Q: RNA Allosteric Transduction for Fluorescence Imaging of Dopamine Modulation in Living Systems.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Apr 4, 2026

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

10.4K

Electrocontrolled Injection-Coupled Droplet Microfluidic Platform for Antimicrobial Resistance Screening.

Wanyi Li1, Hao Zhang1, Chao Hu1

  • 1College of Chemistry and Pharmacy, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.

Analytical Chemistry
|April 3, 2026
PubMed
Summary
This summary is machine-generated.

This study presents a droplet microfluidic platform for rapid antibiotic susceptibility testing (AST). The system precisely encapsulates single bacteria, enabling faster and more accurate antimicrobial resistance detection.

More Related Videos

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
13:42

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

12.7K
Stress-induced Antibiotic Susceptibility Testing on a Chip
12:41

Stress-induced Antibiotic Susceptibility Testing on a Chip

Published on: January 8, 2014

7.0K

Related Experiment Videos

Last Updated: Apr 4, 2026

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

10.4K
Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
13:42

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

12.7K
Stress-induced Antibiotic Susceptibility Testing on a Chip
12:41

Stress-induced Antibiotic Susceptibility Testing on a Chip

Published on: January 8, 2014

7.0K

Area of Science:

  • Biotechnology
  • Microfluidics
  • Molecular Biology

Background:

  • Droplet microfluidics offers high-throughput single-cell analysis.
  • Current methods face challenges in controlling single-bacterium encapsulation for antibiotic susceptibility testing (AST).

Purpose of the Study:

  • To develop an integrated droplet microfluidic platform for precise single-bacterium encapsulation and rapid AST.
  • To enable efficient intradroplet reagent delivery and dual-fluorescence monitoring for bacterial viability and metabolic activity.

Main Methods:

  • Electrocontrolled injection for precise bacterial encapsulation.
  • Optimization of flow parameters for monodisperse droplet generation and stability.
  • Pressure-volume calibration and electroinjection threshold for accurate volume regulation.
  • Coencapsulation of Escherichia coli, pGLO plasmid, and resazurin for dual-fluorescence monitoring (GFP and resorufin).

Main Results:

  • Achieved controllable single-bacterium encapsulation.
  • Demonstrated highly monodisperse droplets with stable morphology.
  • Completed AST within 3 hours via fluorescence readout.
  • Realized dual-fluorescence monitoring indicating bacterial viability and metabolic activity.

Conclusions:

  • The developed multichannel droplet microfluidic system provides a robust and efficient tool for rapid antimicrobial resistance detection.
  • The platform integrates miniaturization, high throughput, and low reagent consumption.
  • Shows considerable potential for clinical translation in AST.