Related Experiment Video
Updated: Apr 5, 2026
Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles
Published on: November 17, 2018
Protocol for immunomagnetic enrichment of CD8 T cells from complex murine tissues
Ella Trolio1, Lelinh Duong1, Michaela Lucas2
1Medical School, University of Western Australia, Perth, WA 6009, Australia.
Efficient isolation of CD8 T cells is essential for accurately investigating their roles in immune regulation and disease pathogenesis. Here, we present a protocol for the isolation and enrichment of CD8 T cells by positive selection from complex murine tissues, including bone marrow (BM), liver, heart, and kidneys. We describe steps for tissue harvesting and processing into single-cell suspensions. We then detail procedures for immunomagnetic CD8 T cell enrichment and assessment of cell purity and viability by flow cytometry.
Efficient isolation of CD8 T cells is essential for accurately investigating their roles in immune regulation and disease pathogenesis. Here, we present a protocol for the isolation and enrichment of CD8 T cells by positive selection from complex murine tissues, including bone marrow (BM), liver, heart, and kidneys. We describe steps for tissue harvesting and processing into single-cell suspensions. We then detail procedures for immunomagnetic CD8 T cell enrichment and assessment of cell purity and viability by flow cytometry.
