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Updated: Apr 5, 2026

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
Published on: July 4, 2016
Di-His-Cu(II)-NTA spin labels for probing protein dynamics: Support from a combined crystallographic/EPR study
Giarita Ferraro1, Eugenio Garribba2, Antonello Merlino1
1Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario di Monte Sant'Angelo, via Cintia, 80126 Naples, Italy.
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In this issue of Structure, Besaw et al.1 report a well-designed study combining electron paramagnetic resonance measurements and structural analyses of di-histidine-Cu(II)-nitrilotriacetate binding sites in T4 lysozyme. The gained insights could have broad implications for the use of double electron-electron resonance spectroscopy in the elucidation of subtle protein conformational variations.
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