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Related Concept Videos

¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

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A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...
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¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

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At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
1.4K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

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Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
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Related Experiment Video

Updated: Apr 7, 2026

Optimization of the Ugi Reaction Using Parallel Synthesis and Automated Liquid Handling
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Integrated Reaction Monitoring and In Situ Structural Elucidation of Reactive Intermediates With Time-Resolved NOAH

Sara M Guzman1, Yael Ben-Tal1, Jiayu Zhang1

  • 1Department of Chemistry, The University of British Columbia, Vancouver, British Columbia, Canada.

Magnetic Resonance in Chemistry : MRC
|April 5, 2026
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Summary

This study introduces a new NMR method for real-time chemical reaction analysis. It allows for rapid structural identification of intermediates and side products, aiding mechanistic studies.

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Area of Science:

  • Organic Chemistry
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Structural elucidation of reaction intermediates is crucial for understanding chemical mechanisms.
  • Isolating these transient species is often difficult, time-consuming, and technically challenging.

Purpose of the Study:

  • To develop a novel Nuclear Magnetic Resonance (NMR) experimental workflow for simultaneous kinetic and structural analysis of active chemical reactions.
  • To enable rapid characterization of reaction intermediates and side products without isolation.

Main Methods:

  • Implementation of a time-resolved Nuclear Magnetic Resonance (NMR) approach using NOAH supersequences.
  • Application of time-resolved NOAH non-uniform sampling (TR-NOAH-NUS) for rapid acquisition of multiple 2D NMR spectra (HSQC, HMBC, COSY).
  • Demonstration on an organo-catalyzed acylation of alcohols within a 30-minute reaction timeframe.

Main Results:

  • Achieved high-quality NMR spectra with temporal resolution under 15 seconds.
  • Successfully generated concentration-time plots for kinetic analysis.
  • Identified and assigned structures of an on-cycle catalytic intermediate and an off-cycle catalyst degradation product.

Conclusions:

  • The TR-NOAH-NUS method provides efficient structural elucidation of transient species in active reactions.
  • This technique facilitates mechanistic understanding by enabling parallel kinetic and structural characterization.
  • Eliminates the need for laborious isolation of intermediates and side products.