Research progress and applications of gene activation editing technology in crops

In recent years, CRISPR/Cas gene editing technology has become a fundamental method in biological breeding. As a vital tool for overcoming technological obstacles, it is currently widely used in functional gene research and genetic enhancement across a variety of organisms. Currently, CRISPR activation (CRISPRa) technology based on dCas9 fusion transcription activation domains has emerged as a powerful tool for expanding the application of CRISPR/Cas systems in improving traits in plants, animals, and microorganisms. This overview starts by going over the underlying principles and components of gene activation editing technology, as well as the phases of development of its three generations. It summarises the present difficulties and potential directions in this field while concentrating on the use of gene activation editing in important crop traits including growth and development regulation, stress resistance, and quality regulation. The objective is to offer valuable insights for the research and development of crop breeding.