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Published on: April 4, 2018
Apolipoprotein E proteotyping as a valid alternative to genotyping in clinical practice
José Manuel Alcalá Ramírez Del Puerto1, Isabel Ortega-Madueño2, María José Gil-Moreno1
1Department of Neurology. Hospital Clínico San Carlos. San Carlos Health Research Institute (IdISSC), Universidad Complutense de Madrid, Madrid, Spain.
None:
The apolipoprotein E ε4 allele (APOE ε4) is associated with higher incidence of amyloid-related imaging abnormalities in patients with Alzheimer's disease undergoing anti-amyloid therapy, underscoring the importance of allele status testing. In this study, plasma ApoE4 levels were measured in 70 participants using Lumipulse®G Pan-ApoE and ApoE4 assays and compared with traditional genotyping. ApoE4 concentrations and ratio ApoE4/Pan-ApoE differed significantly across non-ε4, ε3/ε4, and ε4/ε4 groups. No correlation was observed with age, sex, creatinine levels, or AD cerebrospinal fluid biomarkers. Diagnostic performance was excellent, indicating that proteotyping of ApoE can accurately classify the main APOE haplotypes.
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