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Updated: Apr 8, 2026

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
Published on: March 3, 2010
Phosphorylated Chemiluminescent Probe Based on Spiro-cyclobutyl-phenoxy-dioxetane for the In Situ Light-Activated
Cheng-Jun Zhao1, Wen-Zhen Gui1, Tian-Hao Wang1
1Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai 200237, China.
Abstract:
Alkaline phosphatase (ALP) plays important biological roles for many living species, including bacteria. Here, we developed an ALP probe (CL-A1) using the dephosphorylation of an in situ light-activated chemiluminescent species based on a spiro-cyclobutane-substituted dioxetane scaffold. A sequential light-induced oxidation and ALP-mediated dephosphorylation of CL-A1 rapidly generated intense chemiluminescence (CL), which was stronger than that of the commercial CL substrates AMPPD and APS-5 tested in the presence of a signal enhancer. Analysis using Escherichia coli strains confirmed the applicability of CL-A1 for the sensitive detection of endogenous ALP activity using in situ light activation.
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