Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of Classification and Identification01:28

Methods of Classification and Identification

1.9K
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
1.9K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

7.7K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.7K
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

45
Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
45
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

2.6K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Solvothermal Synthesis of Indium Tin Oxide (ITO) Nanoparticles: From Infrared Localized Surface Plasmon Resonance Absorption to Spontaneous Antibacterial Properties.

Inorganic chemistry·2026
Same author

Submicron Size C@TiO<sub>2</sub> with Multiple Resonance Effects for Surface-Enhanced Raman Spectroscopy and Photocatalysis.

ACS applied materials & interfaces·2026
Same author

Precise Analysis of Surface Adsorption Process on TiO<sub>2</sub>/H<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> during Photocatalytic Degradation through UV-Vis/SERS Technique.

Inorganic chemistry·2026
Same author

Function-Customized Heterostructure for Boosting Semiconductor Surface-Enhanced Raman Scattering and Serving as a Multifunctional Platform.

ACS nano·2025
Same author

SERS-Nanozyme Cooperative Ag@Lacunary-POM Nanoclusters for Exosome Biosensing.

ACS sensors·2025
Same author

Insights into interfacial water and key intermediates on Cu<sub>95</sub>Co<sub>5</sub> aerogels for electrocatalytic nitrate-to-ammonia conversion.

Chemical science·2025
Same journal

Aptamer-based CRISPR-Cas12a fluorescent biosensors for serum biomarker detection.

The Analyst·2026
Same journal

A two-step centrifugal microfluidic platform for semi-automated IGRA detection of tuberculosis based on chemiluminescence.

The Analyst·2026
Same journal

On-site rapid identification of animal and plant creams <i>via</i> 2D FeB nanozyme-based colorimetric sensors.

The Analyst·2026
Same journal

Sensitive detection of aflatoxin B1 using a dual-mode fluorescent aptasensor based on cascade signal amplification.

The Analyst·2026
Same journal

Deep learning-enabled microfluidic digital PCR platform for efficient seven-color quantification.

The Analyst·2026
Same journal

Monitoring food spoilage biogenic amines utilizing a blue-emitting fluorescent ionic liquid.

The Analyst·2026
See all related articles

Related Experiment Video

Updated: Apr 8, 2026

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
12:08

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

3.5K

State-of-the-art of surface-enhanced Raman spectroscopy for pathogenic bacterial identification.

Juntian Jiang1,2, Chao Yan1, Sibo Wang1

  • 1Department of Laboratory Medicine, China-Japan Union Hospital of Jilin University, Jilin University, Changchun 130033, China. xuxs@jlu.edu.cn.

The Analyst
|April 7, 2026
PubMed
Summary
This summary is machine-generated.

Surface-enhanced Raman spectroscopy (SERS) offers a rapid, sensitive, and cost-effective method for identifying pathogenic bacteria. This advanced technique, leveraging molecular fingerprinting, is poised to revolutionize diagnostics in healthcare, food safety, and environmental monitoring.

More Related Videos

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
06:34

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

21.1K
Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging
06:19

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

2.4K

Related Experiment Videos

Last Updated: Apr 8, 2026

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
12:08

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

3.5K
Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
06:34

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

21.1K
Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging
06:19

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

2.4K

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Biotechnology

Background:

  • Current pathogenic bacterial identification methods face challenges including long processing times, high costs, and complexity.
  • Limitations of existing techniques hinder rapid, sensitive, and user-friendly bacterial detection across various applications.
  • There is a critical need for advanced methodologies to meet the growing demand for efficient bacterial identification.

Purpose of the Study:

  • To review recent advancements in Surface-enhanced Raman spectroscopy (SERS) for pathogenic bacterial identification.
  • To highlight breakthroughs in SERS substrate fabrication, sample enrichment, and strain discrimination.
  • To examine the integration of artificial intelligence (AI) and machine learning (ML) with SERS for enhanced bacterial detection.

Main Methods:

  • Systematic review of 126 articles from leading scientific publishers (ACS, Elsevier, Wiley).
  • Analysis of SERS applications in clinical diagnostics, food safety, environmental monitoring, and agriculture.
  • Evaluation of SERS-based detection in complex biological samples like blood, urine, and sputum.

Main Results:

  • SERS demonstrates high sensitivity, rapid measurement, and molecular fingerprinting capabilities for bacterial identification.
  • Key progress has been made in substrate design, sample preparation, and achieving precise strain discrimination using SERS.
  • AI and ML show transformative potential in improving SERS-based bacterial identification accuracy and efficiency.
  • SERS detection in complex clinical specimens shows improved diagnostic sensitivity and specificity.

Conclusions:

  • SERS presents a promising next-generation platform for rapid, portable, and sensitive pathogenic bacterial identification.
  • The integration of AI/ML and advancements in SERS technology are crucial for future diagnostic applications.
  • SERS offers a viable solution to overcome the limitations of conventional methods in public health and safety.
  • Standardization and further development are key for widespread adoption of SERS in routine diagnostics.