Biolipid Film-Fused Electrochemiluminescence for Multipurpose In Situ Bioassays

Area of Science:

• Biotechnology
• Analytical Chemistry
• Materials Science

Background:

• Electrochemiluminescence (ECL) is sensitive to surface states, but its integration with soft biomembranes is underexplored.
• Developing advanced biointerfaces is crucial for understanding membrane dynamics and detecting biomolecules.

Purpose of the Study:

• To create a membrane-interactive scaffold for ECL-emissive artificial nanochannels.
• To enable label-free profiling of cytomembrane-active species and cellular processes.

Main Methods:

• Constructing a biolipid-bound scaffold integrated with ECL-emissive nanochannels and supported phospholipid bilayers.
• Utilizing real-time isotherm and kinetic analysis for profiling.
• Integrating phospholipid-pendant recognition motifs for biomarker detection.
• Employing vesicle-mediated membrane merging for single-cell visualization.

Main Results:

• The biointerface preserves ECL emission and allows dynamic membrane remodeling.
• Achieved label-free profiling of divalent cations, pharmaceuticals, and peptides.
• Demonstrated femtomolar detection of Alzheimer's disease biomarkers (Aβ and tau).
• Enabled single-cell visualization in living cells using endogenous oxygen.

Conclusions:

• The developed biointerface-compatible ECL paradigm facilitates multiplexed monitoring of molecular, membranous, and cellular events.
• This approach offers a versatile platform for studying membrane-associated biological processes.