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Updated: Apr 9, 2026

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots
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An All-in-One Microfluidic Platform for POCT-Ready Nucleic Acid Extraction from Heterogeneous Clinical Samples.

Zhongfu Chen1, Jiali Cao2, Bukong Cui1

  • 1State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, School of Public Health, Xiamen University, Xiamen 361102, China.

Analytical Chemistry
|April 8, 2026
PubMed
Summary
This summary is machine-generated.

A novel universal automated nucleic acid extraction (UNEX) system overcomes limitations in point-of-care diagnostics. It achieves rapid, high-recovery nucleic acid extraction from samples using innovative microfluidics and ultrasonic technology.

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Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Microfluidics

Background:

  • Inhibitors in liquid residue within microfluidic chips hinder automated nucleic acid extraction (NAE) performance in point-of-care (POC) diagnostics.
  • Surfactants, while crucial for lysis, contribute significantly to undesired liquid retention, impacting extraction efficiency.

Purpose of the Study:

  • To develop a multifaceted strategy to overcome liquid residue limitations in automated NAE for POC diagnostics.
  • To engineer a novel microfluidic system for rapid and efficient nucleic acid extraction.

Main Methods:

  • Designed a U-shaped microchannel to reduce liquid retention by 2-fold compared to square channels.
  • Implemented a water-rinse stage to decrease residue by an additional 5-fold, mitigating surfactant effects.
  • Integrated ultrasonic excitation to enhance process efficiency, achieving a 5-minute sample-to-pure-nucleic acid time.

Main Results:

  • Developed the Universal Automated Nucleic Acid Extraction (UNEX) system, a sample-in-to-result-out platform with cold-chain independent, lyophilized reagents.
  • Achieved nucleic acid recovery rates comparable to conventional methods in microchips.
  • Demonstrated exceptional precision (CV < 2%) and quantitative accuracy (R² > 0.99) using various clinical samples.

Conclusions:

  • A holistic approach combining optimized channel geometry, intelligent fluidic protocols, and energy-assisted elution is key to advancing LOC technology in diagnostics.
  • The UNEX system offers a robust, field-deployable solution for automated nucleic acid extraction, enhancing POC molecular diagnostics.