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Related Experiment Video

Updated: Apr 10, 2026

A Polyaniline-based Sensor of Nucleic Acids
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A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

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Homogeneous PDA/Fe3O4 Nanocomposites and 3D-HCR/AgNCs-Based One-Pot Biosensor for Triple-Modal Nucleic Acid

Yingjing Li1, Yi Wang1, Dan Zhang1

  • 1Provincial Key Laboratory of Multimodal Perceiving and Intelligent Systems, Engineering Research Center of Intelligent Human Health Situation Awareness of Zhejiang Province, Jiaxing Key Laboratory of Molecular Recognition and Sensing, College of Biological and Chemical Engineering, Jiaxing University, Jiaxing, 314001, P. R. China.

Analytical Chemistry
|April 9, 2026
PubMed
Summary

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This study presents a novel trimodal biosensor for accurate nucleic acid detection. The platform integrates electrochemical, fluorescence, and spectroscopy methods for enhanced reliability and sensitivity.

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Integrating multiple detection modes into a single biosensing platform is challenging.
  • Existing methods often require complex procedures and multiple steps.
  • Ultrasensitive and reliable nucleic acid detection is crucial for diagnostics.

Purpose of the Study:

  • To develop a trimodal biosensor for ultrasensitive and precise nucleic acid detection.
  • To integrate electrochemical, fluorescence, and spectroscopy signaling into one platform.
  • To enhance assay reliability through cross-verification of results from multiple detection modes.

Main Methods:

  • Fabrication of a trimodal biosensor using polydopamine (PDA)/Fe3O4/capture probe (CP) nanocomposites and 3D-hybridization chain reaction/silver nanoclusters (3D-HCR/AgNCs).

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Last Updated: Apr 10, 2026

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  • Utilizing the KRAS gene segment to initiate HCR, forming a magnetically separable complex.
  • Employing DNA-templated AgNCs for signal generation across electrochemical, fluorescence, and UV-Vis spectroscopy modes.

    • Main Results:

    • Successful development of a one-pot, trimodal biosensing platform for nucleic acid detection.
    • Demonstrated magnetic separation of the HCR-amplified complex.
    • Achieved enhanced sensitivity, particularly in fluorescence mode, due to aggregation-induced emission enhancement.

    Conclusions:

    • The developed trimodal biosensor offers a facile and efficient approach for integrated sensing.
    • This strategy significantly enhances the reliability and sensitivity of nucleic acid detection.
    • The work paves the way for constructing advanced, multi-modal sensing platforms.