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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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Related Experiment Video

Updated: Mar 10, 2026

Measuring Lactase Enzymatic Activity in the Teaching Lab
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Cytochemical electron microscopic localization o esterase activity in Lactobacillus casei.

J P Brown

    Applied Microbiology
    |June 1, 1970
    PubMed
    Summary

    Researchers localized thiolacetic acid-hydrolyzing enzymes in Lactobacillus casei using lead sulfide precipitation. These enzymes were found at the plasma membrane and cell wall surface, aiding in understanding bacterial enzyme activity.

    Area of Science:

    • Microbiology
    • Enzymology
    • Cell Biology

    Background:

    • Lactobacillus casei is a probiotic bacterium with various enzymatic activities.
    • Understanding enzyme localization is crucial for elucidating cellular functions and metabolic pathways.

    Purpose of the Study:

    • To investigate the localization of enzymes responsible for hydrolyzing thiolacetic acid in Lactobacillus casei.
    • To identify the specific cellular compartments where these enzymes are active.

    Main Methods:

    • Enzyme activity was detected using thiolacetic acid as a substrate.
    • Lead sulfide (PbS) precipitation was employed for in vivo localization.
    • Electron microscopy was used to visualize the PbS deposits.

    Main Results:

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    • Enzymes capable of hydrolyzing thiolacetic acid were successfully localized in two strains of Lactobacillus casei.
    • Electron-opaque PbS deposits, indicating enzyme activity, were primarily observed at the plasma membrane.
    • PbS deposition was also noted on the outer surface of the cell wall.

    Conclusions:

    • The study successfully localized thiolacetic acid-hydrolyzing enzymes in Lactobacillus casei.
    • These enzymes are predominantly associated with the plasma membrane and cell wall.
    • This finding contributes to the understanding of enzyme function and location within Lactobacillus species.