Related Experiment Video
Updated: Apr 11, 2026

High-resolution Episcopic Microscopy HREM - Simple and Robust Protocols for Processing and Visualizing Organic Materials
Published on: July 7, 2017
Damage-limited resolution for X-ray and electron microscopy of organic specimens
1Physics Department, University of Alberta, Edmonton, AB T6G 2E1, Canada.
Abstract:
Analytical expressions for the damage-limited resolution (DLR) are developed and applied to X-ray and electron imaging of beam-sensitive specimens, allowing for variation of the characteristic radiation dose with spatial resolution. The dependence of DLR on specimen thickness is illustrated for the common modes of X-ray and transmission electron-microscope imaging. Similarities and differences between the radiolysis damage caused by electrons and X-rays are discussed. The meaning of a `Bragg boost' in diffracted intensity is discussed.
More Related Videos
Related Concept Videos
Overview of Electron Microscopy
Preparation of Samples for Electron Microscopy
X-ray Diffraction of Biological Samples
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are scattered by the electron clouds around the sample atoms. The X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Super-resolution Fluorescence Microscopy
Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

