Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pharmacodynamic interaction between trimethoprim and sulphonamides for A. pleuropneumoniae: synergistic ratios, optimal potentiation, and dynamic responses assessed via time-kill curves.

Journal of applied microbiology·2026
Same author

Semi-mechanistic PK/PD modeling of enrofloxacin against sensitive Pasteurella multocida: inter-strain variability and prediction of efficacy in pigs.

Journal of applied microbiology·2026
Same author

Pore size dynamics control complex volume swelling in pyroptosis.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Minimum inhibitory concentrations of sulfonamides and trimethoprim for veterinary pathogens: New data for old antibiotics.

Veterinary microbiology·2025
Same author

Population pharmacokinetic modeling of sulfadimethoxine, sulfadiazine and sulfamethoxazole combined to trimethoprim in pigs.

The veterinary quarterly·2025
Same author

Presence of antimicrobial residues in edible pig tissues after exposure to cross-contaminated feed: a case study with sulphadimethoxine/trimethoprim.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment·2025

Related Experiment Video

Updated: Apr 13, 2026

Mechanical Mapping of Spheroids Using Brillouin Spectroscopy
08:27

Mechanical Mapping of Spheroids Using Brillouin Spectroscopy

Published on: December 12, 2025

1.5K

Separating Water Content from Network Dynamics in Cell Nuclei with Brillouin Microscopy.

Lucie Vovard1, Alexis Viel1, Estelle Bastien1

  • 1Institut Lumière Matière, Universite Claude Bernard Lyon 1, CNRS, UMR5306, F-69100 Villeurbanne, France.

Physical Review Letters
|April 11, 2026
PubMed
Summary

Mechanobiology research uses Brillouin light scattering (BLS) to measure cell properties. This study clarifies BLS interpretation by linking sound velocity to water content and linewidth to biopolymer network dynamics in cells.

More Related Videos

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy
10:57

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

Published on: November 11, 2025

994
Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method
07:00

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method

Published on: June 24, 2020

27.3K

Related Experiment Videos

Last Updated: Apr 13, 2026

Mechanical Mapping of Spheroids Using Brillouin Spectroscopy
08:27

Mechanical Mapping of Spheroids Using Brillouin Spectroscopy

Published on: December 12, 2025

1.5K
Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy
10:57

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

Published on: November 11, 2025

994
Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method
07:00

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method

Published on: June 24, 2020

27.3K

Area of Science:

  • Cellular mechanics
  • Biophysics
  • Mechanobiology

Background:

  • Cellular mechanical properties are crucial in mechanobiology.
  • Traditional methods involve deforming cells and measuring forces.
  • Brillouin light scattering (BLS) offers an alternative by measuring acoustic parameters on picosecond timescales.

Purpose of the Study:

  • To clarify the interpretation of hypersonic data from Brillouin light scattering (BLS) spectra.
  • To differentiate contributions of water content and biopolymer network dynamics to cellular mechanical properties.
  • To resolve confusion arising from the colloquial use of 'stiffness' in cell mechanics.

Main Methods:

  • Applied osmotic compressions to single cells to vary intracellular water and solid network fractions.
  • Utilized Brillouin light scattering (BLS) to measure sound velocity and attenuation.
  • Compared BLS data with cell volume measurements and fitted results to a poroelastic model.

Main Results:

  • Observed a nonlinear increase in sound velocity and attenuation in the nucleus with increasing osmotic pressure.
  • Demonstrated that BLS shift is primarily sensitive to intracellular water content.
  • Showed that BLS linewidth analysis reveals contributions from biopolymer network dynamics.

Conclusions:

  • The study successfully differentiates between water content and biopolymer network contributions using BLS.
  • Poroelastic modeling aids in interpreting hypersonic data from BLS.
  • This work provides a clearer understanding of BLS applications in living cell mechanics.