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Reactive oxygen species (ROS) enhance proton exchange rate (kex) in a small-metabolite CEST system
1Radiology Department, University of Illinois at Chicago, Chicago, IL, USA.
Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|April 12, 2026
Summary
Reactive oxygen species (ROS) can be detected noninvasively using chemical exchange saturation transfer (CEST) MRI. This study shows ROS increase proton exchange rates, supporting CEST MRI as a biomarker for oxidative stress.
Area of Science:
- Biomedical Imaging
- Biomarkers
- Oxidative Stress Research
Background:
- Reactive oxygen species (ROS) are crucial biomarkers for oxidative stress, linked to neurodegeneration and cancer.
- Noninvasive ROS imaging is challenging due to low concentrations and transient nature.
- Previous work showed ROS enhance proton exchange rates (kex) in CEST MRI of egg whites.
Purpose of the Study:
- To validate ROS-induced kex changes in a simpler system.
- To confirm kex as a reliable indicator for ROS detection using MRI.
Main Methods:
- Utilized 9.4T chemical exchange saturation transfer (CEST) Z-spectral MRI.
- Employed Bloch-McConnell modeling for data analysis.
- Used creatine solutions with Fenton reactions to generate ROS.
Main Results:
- ROS significantly broadened creatine CEST spectra.
- Broadening was primarily attributed to increased proton exchange rates (kex).
- No significant changes in relaxation rates were observed.
Conclusions:
- Endogenous kex mapping via CEST MRI is a promising method for in vivo ROS detection.
- This approach can aid in managing oxidative stress-related diseases.
- Further supports CEST MRI's potential as a noninvasive biomarker.
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