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The lipid flippase MoNeo1 mediates vesicle trafficking and pathogenicity in Magnaporthe oryzae

Yan Cai1, Xiuwei Huang1, Yufan Nie1

  • 1State Key Laboratory of Agricultural and Forestry Biosecurity & Key Lab of Biopesticide and Chemical Biology, Ministry of Education, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Stress Biology
|April 13, 2026
PubMed

Insights

The P4-ATPase flippase MoNeo1 is crucial for the rice blast fungus Magnaporthe oryzae's growth and infection. It regulates lipid homeostasis and vesicle transport, impacting pathogenicity.

Area of Science:

  • Plant pathology
  • Molecular biology
  • Mycology

Background:

  • The rice blast fungus Magnaporthe oryzae is a significant threat to global rice production.
  • Understanding the molecular mechanisms underlying fungal development and pathogenicity is critical for disease control.

Purpose of the Study:

  • To characterize the role of MoNeo1, a P4-ATPase flippase, in the pathogenicity of Magnaporthe oryzae.
  • To investigate the relationship between MoNeo1, lipid homeostasis, and vesicle transport in the fungus.

Main Methods:

  • Gene deletion and phenotypic analysis of the MoNEO1 mutant.
  • Lipidome profiling to assess changes in lipid composition.
  • Co-immunoprecipitation and subcellular localization studies to investigate protein interactions and transport pathways.

Main Results:

  • Deletion of MoNEO1 severely impaired fungal growth, conidiation, appressorium formation, and virulence.
  • MoNeo1 is essential for maintaining lipid homeostasis, with mutants showing altered levels of various phospholipids and storage lipids.
  • MoNeo1 interacts with the retromer component MoVps35 and its localization depends on retromer-mediated transport.
  • MoNeo1 is required for the stability and transport of the SNARE protein MoSnc1, essential for effector secretion.

Conclusions:

  • MoNeo1 acts as a key regulator of fungal development and pathogenicity in Magnaporthe oryzae.
  • The P4-ATPase flippase MoNeo1 integrates lipid dynamics with vesicle transport machinery to facilitate fungal pathogenesis.
  • Targeting MoNeo1 could be a potential strategy for controlling rice blast disease.
Keywords:
Magnaporthe oryzaeFlippasePathogenicityVesicle transport

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