Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

3.3K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
3.3K
Replicative Cell Senescence02:15

Replicative Cell Senescence

3.5K
3.5K
Multipotency and Niche of Bulge Stem Cell01:06

Multipotency and Niche of Bulge Stem Cell

4.4K
A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
4.4K
Introduction to Fibroblasts01:09

Introduction to Fibroblasts

4.8K
Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
4.8K
Tissue Renewal without Stem Cells01:23

Tissue Renewal without Stem Cells

2.3K
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MS-DIAL-based metabolomic profiling of tetrodotoxin and its analogues in toxic marine organisms.

Toxicon : official journal of the International Society on Toxinology·2026
Same author

The Greenland shark genome: Insights into lifespan extremes and population dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Environmental Exosomes/Small Extracellular Vesicles: Evidence of Extracellular RNA Release by Aquatic Organisms.

Marine biotechnology (New York, N.Y.)·2026
Same author

Genome refinement in Pacific bluefin tuna (Thunnus orientalis): chromosome-level assembly using hybrid linkage data.

BMC genomics·2026
Same author

Comparative single-cell and spatial profiling of anti-SSA-positive and anti-centromere-positive Sjögren's disease reveals common and distinct immune activation and fibroblast-mediated inflammation.

Nature communications·2025
Same author

Atypical Femoral Fracture During Long-Term Treatment With Denosumab in Patients With Metastatic Bone Tumors.

Cureus·2025
Same journal

Inhibition of peroxisomal protein PRX-11 promotes longevity in <i>Caenorhabditis elegans</i> via enhancements to mitochondria.

Aging·2026
Same journal

Senescent cells accumulate lipid droplets.

Aging·2026
Same journal

Short-term responsiveness of DNA methylation-based aging biomarkers to a multimodal intervention comprising exercise and dietary guidance involving daily consumption of yogurt containing <i>Bifidobacterium longum</i> BB536: an exploratory randomized controlled trial.

Aging·2026
Same journal

Hormonal dimorphism in sarcopenia disease.

Aging·2026
Same journal

The multifaceted inducers of cellular senescence.

Aging·2026
Same journal

Life expectancy and causes of death in classical laminopathic progeroid syndromes: systematic review with individual-patient data synthesis.

Aging·2026
See all related articles

Related Experiment Video

Updated: Apr 15, 2026

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts
07:03

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

Published on: January 2, 2018

6.7K

Stage-dependent transcriptomic changes in human dermal fibroblast senescence model.

Michiko Kudo1,2, Ryo Yonezawa1, Hideaki Mizobata1

  • 1Laboratory of Aquatic Molecular Biology and Biotechnology, Department of Aquatic Bioscience, Graduate School of Agricultural and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657 Japan.

Aging
|April 13, 2026
PubMed
Summary
This summary is machine-generated.

Cellular senescence involves distinct gene expression changes as cells age. Immune responses activate early, while structural maintenance declines progressively, offering new therapeutic targets for aging.

Keywords:
aging biomarkerscellular senescencedermal fibroblastsimmune–inflammatory signalingtranscriptome analysis

More Related Videos

Induction and Validation of Cellular Senescence in Primary Human Cells
08:18

Induction and Validation of Cellular Senescence in Primary Human Cells

Published on: June 20, 2018

18.4K
Techniques to Induce and Quantify Cellular Senescence
06:51

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

35.6K

Related Experiment Videos

Last Updated: Apr 15, 2026

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts
07:03

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

Published on: January 2, 2018

6.7K
Induction and Validation of Cellular Senescence in Primary Human Cells
08:18

Induction and Validation of Cellular Senescence in Primary Human Cells

Published on: June 20, 2018

18.4K
Techniques to Induce and Quantify Cellular Senescence
06:51

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

35.6K

Area of Science:

  • Cellular biology
  • Aging research
  • Genomics

Background:

  • Cellular senescence is crucial in aging and age-related diseases.
  • The precise gene expression dynamics during senescence are not fully understood.

Purpose of the Study:

  • To investigate gene expression changes throughout aging progression.
  • To identify molecular mechanisms driving senescence.
  • To explore potential biomarkers and therapeutic targets for aging.

Main Methods:

  • Utilized three replicative senescence models of human dermal fibroblasts (young, middle, old).
  • Performed RNA sequencing transcriptome analysis with two analytical pipelines.
  • Conducted differential expression analysis, STRING, Gene Ontology/Kyoto Encyclopedia of Genes and Genomes enrichment, and non-negative matrix factorization (NMF).

Main Results:

  • Transcriptome profiles differed significantly between young/middle and old stages.
  • Gene expression changes (differentially expressed genes) increased with senescence progression.
  • Early activation of immune-inflammatory responses and decreased structural maintenance genes were observed.
  • NMF confirmed immune-inflammatory activation and loss of structural maintenance functions.

Conclusions:

  • Immune-inflammatory responses are activated early in senescence.
  • Cell adhesion and maintenance pathways decline progressively during aging.
  • Identified early biomarkers and therapeutic targets to potentially mitigate aging-related functional decline.