Interactions Between Root Traits and Fungal Functional Guilds Across the Root Economics Spectrum

Soil fungi play a pivotal role in maintaining ecosystem functions and regulating plant health. Although plant root traits can significantly impact the abundance and diversity of different fungal groups, the mechanism by which plant root strategies drive the assembly of soil fungal guilds remains limited. Utilizing Root Economics Space theory, this study investigates how four green manures (hairy vetch, rye, radish, and rapeseed) with contrasting root functional strategies (along the 'fast-slow' and 'outsourcing-DIY' axes) regulate the composition and functional structure of soil fungal communities. Community characteristics of three functional guilds (plant pathogens, saprophytes, and arbuscular mycorrhizal fungi), as well as relationships between these communities and plant root traits, were evaluated using a combination of Illumina high-throughput sequencing, functional annotation, and multivariate statistical analysis. Overall, different root strategies were associated with distinct fungal community patterns, potentially related to differences in root-derived resource inputs and soil properties. The 'slow' and 'DIY' strategies were associated with lower relative abundance of plant pathogenic fungi and higher relative abundance of saprotrophic fungi, whereas the 'fast' and 'outsourcing' strategies were associated with higher relative abundance of plant pathogens and AMF. These findings suggest that root functional strategies may help explain variation in fungal guild composition under different green manure species. From a practical perspective, the results provide a basis for selecting green manure species to help manage soil-borne disease risk, regulate beneficial soil microbial communities, and support more sustainable soil management in agricultural production.