Estimation of Nitrogen Status in Zanthoxylum armatum var. novemfolius Using Machine Learning Algorithms and UAV...

Accurate monitoring of nitrogen (N) status is critical for precision N management and optimizing the yield and quality of Zanthoxylum armatum var. novemfolius (ZA). However, individual sensors often struggle to simultaneously capture the biochemical variations and complex canopy structural changes of ZA. Therefore, field experiments were conducted over two consecutive years, applying four N-application rates (0, 150, 300, and 450 kg N ha^-1) to ZA. At each phenological stage, hyperspectral imagery and LiDAR point clouds were collected via three UAV flight altitudes (60 m, 80 m, and 100 m), and canopy nitrogen concentration (CNC) and aboveground nitrogen accumulation (AGNA) were measured. This study developed a framework by synergistically fusing UAV-derived hyperspectral imaging (HSI) and LiDAR data for CNC and AGNA monitoring. Results showed that the response of nitrogen status indicators to fertilization was phenology-specific: CNC showed no significant difference (p > 0.05) among treatments during the vigorous vegetative growth stage (VGS) but differed significantly (p < 0.05) during the fruit expansion stage (FES); AGNA differed significantly among treatments at VGS and FES (p < 0.05). The two-step screening yielded NDSI _{(732, 879)} and NDSI _{(560, 690)} as the optimal CNC indicators at VGS and FES, respectively (r = 0.83 and 0.93), whereas the NDSI _{(711, 986)} and NDSI _{(515, 736)} were identified as the optimal AGNA indicators at VGS and FES, respectively (r = 0.91 and 0.71). Across all phenological stages, Random Forest Regression consistently delivered the highest accuracy for CNC (R² = 0.93-0.98, RMSE = 0.87-1.02 g kg^-1) and AGNA (R² = 0.95-0.97, RMSE = 1.92-2.55 g plant^-1), outperforming MLR, PLSR, and SVR. This synergistic framework provides a high-precision, non-destructive methodology for the precision N monitoring of woody crops.