Effects of a Mediterranean Diet-Based Program on Cognitive Decline: Non-Blinded Non-Randomized Controlled Trial of...

Background: Age-related cognitive deccline is a significant health issue in Spain, especially among adults over 60 years of age. Addressing this involves establishing intervention guidelines and identifying early diagnostic biomarkers. Objective: To evaluate changes in urine of Brain-Derived Neurotrophic Factor, concentration and cognitive performance after the implementation of the multicomponent CESPORT program (incorporating a Mediterranean Diet, nutritional education, and continuous support). Methods: This controlled trial included 76 older adults, divided into an experimental group (n = 58; mean age 66.9 years; 75.9% female) that participated in the CESPORT program, and a control group (n = 18; mean age 68.8 years; 72.2% female). Cognitive performance was assessed using the Mini-Mental State Examination (MMSE) and the Cognifit^® battery. Urinary BDNF concentrations were quantified via ELISA. Results: After adjusting for baseline scores via ANCOVA, the experimental group demonstrated significantly higher post-intervention outcomes compared to the control group (p < 0.001). Substantial improvements with medium-to-large effect sizes were observed in global cognition, reasoning, attention, coordination and perception. Furthermore, urinary BDNF levels were significantly elevated in the experimental group. Positive correlations were found between Brain-Derived Neurotrophic Factor concentrations and cognitive performance in multiple domains (p < 0.05), particularly regarding global status and reasoning. Conclusions: The multicomponent CESPORT intervention demonstrates a potential protective effect against age-related cognitive decline. Furthermore, urinary BDNF emerges as a promising, non-invasive early biomarker for cognitive health. Further research is warranted to validate these findings.