Related Experiment Video
Updated: Apr 15, 2026
Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker
Published on: March 31, 2018
[Expression and significance of the FABP6 long transcript in clear cell renal cell carcinoma]
Haoming Yin1, Zijie Wang1, Fan Shu1
1Department of Urology, Peking University Third Hospital, Beijing 100191, China.
Objective:
To investigate the expression of the fatty acid binding protein 6 (FABP6) long transcript in clear cell renal cell carcinoma (ccRCC), its correlation with tumor biological behavior, and further analyze its potential as a biomarker and therapeutic target.
Methods:
Following bioinformatics analysis of the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases, the FABP6 gene associated with ccRCC development and prognosis was screened. The existence and expression patterns of FABP6 long and short transcripts were further confirmed experimentally. In the experimental section, reverse transcription quantitative real-time PCR (RT-qPCR) and Western blot were used to detect the differential expression levels of the FABP6 long and short transcripts in ccRCC cell lines and tissue samples. ccRCC cell lines with overexpression and knockdown of the FABP6 long transcript were constructed. The impact of the FABP6 long transcript on the proliferation capacity of ccRCC cells was assessed using the 5-ethynyl-2'-deoxyuridine proliferation assay and the colony formation assay, respectively.
Results:
Bioinformatics database analysis revealed that the expression of the FABP6 gene was higher in ccRCC cell lines and tissue samples compared with their normal counterparts (P=0.02), with FABP6 long transcript being the predominant form (P=0.02). RT-qPCR and Western blot results further confirmed that the expression level of the FABP6 long transcript was higher than that of the FABP6 short transcript in ccRCC cell lines such as 769P, A498, CAKI1, OSRC2, and 786O. In in vitro functional experiments, overexpression of the FABP6 long transcript promoted the proliferation of ccRCC cells. Conversely, knockdown of the FABP6 long transcript significantly inhibited the proliferation of ccRCC cells. This suggested that the FABP6 long transcript might play an oncogenic role in the development and progression of ccRCC, potentially by driving cell cycle progression or regulating related proli-ferative signaling pathways.
Conclusion:
This study systematically reports the specific high expression of the FABP6 long transcript in ccRCC. Gain-of-function and loss-of-function experiments confirmed its crucial role in promoting ccRCC cell proliferation. This reveals an important new function of the FABP6 gene, particularly FABP6 long transcript, in the malignant progression of ccRCC.
