Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An Oscillating-Flow Microfluidic PCR Method for Rapid and Flexible Detection of Periodontal Pathogens.

Sensors (Basel, Switzerland)·2026
Same author

Parameter-Reduced YOLOv8n with GhostConv and C3Ghost for Automated Blood Cell Detection.

Bioengineering (Basel, Switzerland)·2026
Same author

Automated Detection of Parasitic Elements in Veterinary Fecal Samples Using a Deep Learning-Based Object Detection Framework.

Veterinary sciences·2026
Same author

A Brief Review on the Analysis of dsDNA, RNA, Amino Acids and Bacteria by Capillary Electrophoresis.

Bioengineering (Basel, Switzerland)·2025
Same author

Development of a Low-Cost and Easy-Assembly Capillary Electrophoresis System for Separation of DNA.

Bioengineering (Basel, Switzerland)·2025
Same author

High-throughput 3D microfluidic chip for generation of concentration gradients and mixture combinations.

Lab on a chip·2024

Related Experiment Video

Updated: Apr 15, 2026

Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye
07:44

Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye

Published on: November 5, 2010

25.5K

A low-cost, high-throughput DNA quantification system using light pipe arrays for parallel fluorescence measurement.

Jiandong Zhu1, Haojie Sun2, Bo Yang1

  • 1Engineering Research Center of Optical Instrument and System, Key Lab of Optical Instruments and Equipment for Medical Engineering, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China.

Analytical Methods : Advancing Methods and Applications
|April 14, 2026
PubMed
Summary

A new, low-cost system enables high-throughput DNA concentration measurement using fluorescent dyes. This parallel analysis improves accuracy and efficiency for molecular biology experiments.

More Related Videos

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
06:55

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Published on: September 24, 2015

8.8K
Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

23.1K

Related Experiment Videos

Last Updated: Apr 15, 2026

Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye
07:44

Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye

Published on: November 5, 2010

25.5K
Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
06:55

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Published on: September 24, 2015

8.8K
Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

23.1K

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Accurate DNA quantification is crucial for molecular biology.
  • UV absorbance is common but lacks sensitivity for low concentrations.
  • Existing fluorometric methods face challenges like cross-talk and low throughput.

Purpose of the Study:

  • To develop a high-throughput, low-cost, and compact system for DNA concentration measurement.
  • To overcome limitations of traditional single-channel fluorometric designs.

Main Methods:

  • Developed a system with an LED array, optical filters, and eight independent light pipes for simultaneous measurements.
  • Evaluated fluorescence intensity of DNA/dye mixtures (SYBR Green I, GelRed, SYBR Gold) in different buffers (0.5× TBE, 1× TAE, ultrapure water).
  • Determined optimal dye concentrations and assessed system performance using correlation coefficients and fluorescence stability.

Main Results:

  • 0.5× TBE buffer yielded stronger fluorescence than 1× TAE or ultrapure water.
  • Optimal dye concentrations were identified: 3× for GelRed, 8× for SYBR Gold, 25× for SYBR Green I.
  • High correlation coefficients (R > 0.92) were achieved for all dyes, with SYBR Green I showing the best performance (R = 0.972-0.996) and stability (RSD < 2.55%).

Conclusions:

  • The developed system offers a reliable, efficient, and cost-effective platform for parallel DNA concentration analysis.
  • High throughput, stability, and minimized cross-talk reduce measurement time and cost.
  • The system is suitable for routine molecular biology labs and large-scale studies, enhancing experimental accuracy and workflow efficiency.