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Related Concept Videos

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...

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Related Experiment Video

Updated: Jul 5, 2026

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
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Time-resolved transcriptomics of FEN1 knockdown HEK293T cells identifies altered rhythmic gene expression.

Fei Ge1, Lijun Yu2, Yuling Sun3

  • 1Jiangsu Province Engineering Research Center of Development and Translation of Key Technologies for Chronic Disease Prevention and Control, Suzhou Vocational Health College, Suzhou 215009, China; Department of Basic Medicine, Suzhou Vocational Health College, Suzhou 215009, China.

Gene
|April 14, 2026
PubMed
Summary

The DNA repair enzyme FEN1 influences daily gene expression rhythms, impacting cell cycle control and senescence. FEN1 depletion disrupts circadian transcriptional patterns, affecting cellular homeostasis and genome maintenance.

Keywords:
Cell cycleCellular senescenceCircadian rhythmDNA damage responseFlap Endonuclease 1Transcriptome analysis

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Area of Science:

  • Molecular Biology
  • Genetics
  • Chronobiology

Background:

  • Circadian rhythms regulate daily gene expression for cellular homeostasis.
  • The interplay between circadian transcription and DNA damage response/cell cycle regulation is not fully understood.

Purpose of the Study:

  • To investigate the role of the DNA replication and repair nuclease FEN1 in circadian transcriptional dynamics.
  • To explore the connection between FEN1, genome maintenance, and temporal gene expression programs.

Main Methods:

  • Time-series RNA sequencing in synchronized HEK293T cells across six circadian time points.
  • FEN1 knockdown experiments to assess effects on gene expression rhythmicity.
  • Cell-based analyses of cell cycle distribution and senescence markers.

Main Results:

  • FEN1 knockdown altered rhythmicity in approximately 30% of oscillating transcripts, including phase shifts and amplitude changes.
  • Genes with altered rhythmic properties were enriched in cell cycle regulation, DNA damage response, and senescence pathways.
  • FEN1 depletion led to G1 phase accumulation, reduced S-phase entry, and increased senescence markers.

Conclusions:

  • FEN1 plays a role in maintaining circadian transcriptional patterns and regulating cell cycle progression.
  • Disruption of FEN1 impacts genome maintenance pathways and cellular temporal organization.
  • This study provides insights into the links between DNA repair mechanisms and the circadian clock.