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Related Experiment Video

Updated: Apr 16, 2026

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

A Cell-Resolved Ultrastable Biosensor Enables One-Step Detection of Gene-Fusion Transcripts in Unprocessed Whole

Chuipeng Kong¹, Xin Zhang², Yuanyuan Chen³

¹Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Analytical & Testing Centre, Sichuan University, Chengdu, Sichuan, P. R. China.

Angewandte Chemie (International Ed. in English)

|April 15, 2026

Summary

Researchers developed a new sensor for detecting leukemia fusion transcripts in whole blood. This cell-resolved ultrastable sensor for hematology (CRUSH) provides rapid and accurate diagnosis of hematologic malignancies.

Keywords:

Au‐S bonds biosensors cell selectivity defect‐free SAMs stability

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Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

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Last Updated: Apr 16, 2026

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

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Published on: April 16, 2016

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

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Published on: April 5, 2018

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

Area of Science:

Biomedical Engineering
Nanotechnology
Hematology

Background:

Accurate detection of aberrant mRNA in blood is crucial for diagnosing hematological malignancies.
Existing sensors struggle with unprocessed whole blood due to stability and cell-targeting issues.

Purpose of the Study:

To engineer a highly stable, cell-specific sensor for detecting leukemia fusion transcripts in unprocessed whole blood.
To develop a rapid, one-step diagnostic test for hematologic malignancies.

Main Methods:

Spherical nucleic acid (SNA) technology was utilized to create the cell-resolved ultrastable sensor for hematology (CRUSH).
CRUSH features a defect-free thiol monolayer on gold nanoparticles (AuNPs) for enhanced stability.
Selective internalization of CRUSH by myeloid cells was leveraged for cell-specific detection.

Main Results:

CRUSH demonstrated record-breaking stability, withstanding 0.1 M dithiothreitol.
The sensor enabled live-cell detection of BCR-ABL1 fusions in unprocessed whole blood.
A one-step mixed-and-read diagnostic test using CRUSH accurately discriminated multilineage acute lymphoblastic leukemia within 1 hour.

Conclusions:

CRUSH offers a rapid, specific, and robust tool for diagnosing hematologic malignancies.
This biosensing innovation addresses urgent diagnostic needs in hematology.
The technology enables precise detection of fusion transcripts in living myeloid cells.