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Updated: Aug 6, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
Published on: December 29, 2021
An autocatalytic DNA circuit via polymerase mediated strand displacement
Sitong Lv1, Guan Alex Wang1, Feng Li1,2
1Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, Chengdu, Sichuan, 610064, P. R. China. guanalexwang@scu.edu.cn.
None:
Herein, we present an autocatalytic DNA circuit powered by a single polymerase. The autocatalysis was achieved via two displacement layers, where the output of one layer served as the trigger of the other. Each input was designed to release two outputs through polymerase-driven strand displacement. Such a design enabled rapid accumulation of output molecules and characteristic exponential signal amplification via a single enzyme.
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