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Related Experiment Video

Updated: Jun 29, 2026

Visual Detection of Multiple Nucleic Acids in a Capillary Array
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A LEGO-inspired multipiece chip for portable nucleic acid detection.

Ye Peng1, Lu Gao1, Pengkun Yin1

  • 1Key Laboratory of Green Chemistry & Technology of Ministry of Education, College of Chemistry, Sichuan University, Chengdu, Sichuan 610064, China. dongielp3@163.com.

Chemical Communications (Cambridge, England)
|February 25, 2026
PubMed
Summary

We developed a modular microfluidic chip (LIM-Chip) for streamlined nucleic acid amplification and detection. This system enables sensitive HPV16 DNA detection in clinical samples using a DNAzyme assay.

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Area of Science:

  • Biomedical Engineering
  • Molecular Diagnostics
  • Microfluidics

Background:

  • Microfluidic devices offer advantages for nucleic acid amplification and detection.
  • Current modular microfluidic systems can be complex to assemble and customize.

Purpose of the Study:

  • To develop a modular microfluidic chip (LIM-Chip) with on-demand assembly capabilities.
  • To demonstrate the clinical applicability of the LIM-Chip for sensitive pathogen detection.

Main Methods:

  • Designed a LEGO-inspired multipiece chip (LIM-Chip) utilizing 3D-printed connectors for modular assembly.
  • Developed a DNAzyme-based isothermal nucleic acid amplification assay.
  • Applied the LIM-Chip to detect HPV16 DNA in clinical cervical samples.

Main Results:

  • The LIM-Chip allows for rapid and on-demand assembly of microfluidic systems.
  • The system successfully mediated a DNAzyme-based assay for sensitive HPV16 DNA detection.
  • Demonstrated clinical applicability using real-world cervical samples.

Conclusions:

  • The LIM-Chip provides a versatile and user-friendly platform for integrated nucleic acid amplification and detection.
  • This modular approach simplifies the development and application of microfluidic devices in clinical diagnostics.