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Updated: Apr 16, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Base editing in rice using nuclease-deactivated CRISPR/Cas-SF01.

Meng Jia1, Yangqing Xie1, Chuang Wei1

  • 1Key Laboratory of Gene Editing Technologies (Hainan), Ministry of Agriculture and Rural Affairs, National Nanfan Research Institute, and Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Sanya, 572024, China.

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|April 15, 2026
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Summary

Researchers achieved targeted base substitutions in rice using adenine and cytosine base editing. A novel variant expanded base editing capabilities to new DNA sequences, enhancing precision in rice genetic research.

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Area of Science:

  • Plant Molecular Biology
  • Genetics and Genomics
  • Biotechnology

Background:

  • CRISPR-Cas systems have revolutionized genome editing.
  • Base editing offers precise single nucleotide modification without double-strand breaks.
  • Efficient base editing in rice is crucial for crop improvement.

Purpose of the Study:

  • To evaluate the efficacy of dCas-SF01 for adenine and cytosine base editing in rice.
  • To develop and test a novel dCas-SF01 variant for expanded PAM compatibility.
  • To assess the efficiency of base editing at multiple loci in rice.

Main Methods:

  • Utilized the dCas-SF01 base editor and the 35S-CmYLCV-U6 composite promoter.
  • Introduced targeted base substitutions at multiple genomic sites in rice.
  • Employed a protospacer adjacent motif (PAM)-relaxed SF01-IKRR variant for broader PAM recognition.

Main Results:

  • Achieved successful targeted base substitutions in rice with an average editing efficiency of 33.3%.
  • Demonstrated the capability of dCas-SF01 for both adenine and cytosine base editing.
  • The SF01-IKRR variant enabled base editing with 5'-NTN PAMs, significantly expanding targeting scope.

Conclusions:

  • dCas-SF01 is an effective tool for precise base editing in rice.
  • The PAM-relaxed variant broadens the applicability of base editing in rice genome engineering.
  • These advancements hold promise for accelerating rice genetic improvement and functional genomics studies.