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A key role for S-nitrosylation in immune regulation and development in the liverwort Marchantia polymorpha
Nadra Tabassum1,2, Justin Goodrich1, Gary J Loake1
1Institute of Molecular Plant Sciences, School of Biological Sciences, Edinburgh University, Edinburgh EH9 3BF, UK.
Journal of Experimental Botany
|April 15, 2026
Summary
Nitric oxide (NO) is crucial for plant immunity. Researchers studied the S-nitrosoglutathione reductase 1 (GSNOR1) gene in Marchantia liverworts, finding it vital for development and immune responses via S-nitrosylation.
Area of Science:
- Plant biology
- Molecular genetics
- Biochemistry
Background:
- Nitric oxide (NO) acts as a key signaling molecule in flowering plant immunity.
- NO regulates plant defense mechanisms through S-nitrosylation, a redox-dependent modification of cysteine residues.
Purpose of the Study:
- To investigate the function of the S-nitrosoglutathione reductase 1 (MpGSNOR1) gene in Marchantia polymorpha.
- To understand the role of S-nitrosylation in a basal land plant lineage.
Main Methods:
- CRISPR/Cas9 genome editing was used to create loss-of-function alleles of MpGSNOR1.
- Morphological and molecular analyses were performed on mutant lines.
Main Results:
- Disruption of MpGSNOR1 led to significant morphological alterations in Marchantia.
- MpGSNOR1 was found to be essential for maintaining S-nitrosothiol (SNO) homeostasis.
- The gene plays a critical role in Marchantia's immune function.
Conclusions:
- GSNOR1 is integral to both structural development and immune responses in Marchantia.
- The findings suggest that GSNOR was a conserved component in ancestral land plants, involved in immunity and development.
Keywords:
Marchantia polymorphaS-nitrosylationEvoMPMIGSNORnitric oxideplant immunityreactive nitrogen speciesredoxMore Related Videos
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