Quantitative evaluation of Kalayanak Ghrita and Sarasvat Ghrita dosage using a Drosophila life-history framework

Ayurveda, an ancient Indian system of medicine, is recognized for its holistic therapeutic approach; however, many supplementations lack experimental, dose-defined validation. Here, we examine the biological impact and optimal dosage of Ayurvedic Rasayana, Kalyanaka Ghrita and Sarasvata Ghrita, using Drosophila melanogaster as model organism. By analysing key life-history traits-development, fecundity, and longevity-we show that neither supplementation affects development, while higher concentrations negatively influence survival and reproductive output. Lower doses are largely well tolerated, defining a safe physiological window. This study provides quantitative insight into Ayurvedic dosage effects and demonstrates the utility of Drosophila for physiological evaluation of traditional medicines.