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Updated: Apr 18, 2026
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Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue
Published on: June 21, 2018
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Continuous Sensing of Lactate Dehydrogenase with a Competitive Particle-Based Sensor for Monitoring Cell Death.
Ekaterina Belina1,2, Arthur M de Jong2,3, Menno W J Prins1,2,3,4
1Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven 5600 MB, the Netherlands.
ACS Sensors
|April 16, 2026
Summary
This study introduces a novel sensor for continuous cell death monitoring by detecting lactate dehydrogenase (LDH). The Biosensing by Particle Motion technology offers real-time, minimally invasive insights into cell viability dynamics.
Area of Science:
- Biomedical Engineering
- Cell Biology
- Analytical Chemistry
Background:
- Continuous monitoring of cell death is crucial for understanding biosystems and maintaining cell viability.
- Current methods for cell death detection are often invasive, provide single-point data, and limit long-term studies.
Purpose of the Study:
- To develop a minimally invasive, time-resolved sensing technology for continuous detection of lactate dehydrogenase (LDH).
- To establish a foundation for reagent-free sensors for monitoring cell death in living biosystems.
Main Methods:
- Development of a Biosensing by Particle Motion (BPM) sensor for LDH detection.
- Utilized an affinity-based competitive assay with LDH analogue on particles and anti-LDH antibodies on a surface.
- Screened antibody candidates for reversible binding kinetics to ensure dynamic response.
Main Results:
- Achieved nanomolar detection range for LDH with minute-scale response times.
- Demonstrated sensor functionality in both buffer and cell culture medium.
- Validated the feasibility of continuous LDH sensing using the BPM technology.
Conclusions:
- The developed BPM sensor enables continuous, minimally invasive monitoring of cell membrane disruption via LDH detection.
- This technology provides a foundation for reagent-free, time-resolved biosensing applications.
- Potential applications include bioprocess control, drug screening, toxicity testing, and biomedical research.
