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Updated: Apr 19, 2026

Enzymatic Cascade Reactions for the Synthesis of Chiral Amino Alcohols from L-lysine
Published on: February 16, 2018
Engineering Ene-Reductases for the Chemoenzymatic Synthesis of a Sacubitril Intermediate and Its Derivatives
Jingshuan Zhou1,2, Xinghua Wang1,2, Yikai Wang2
1College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, P. R. China.
Abstract:
The availability of an effective ene-reductase (ER) for the synthesis of Sacubitril intermediates represents a major challenge. Herein, we report the engineering of ClER and GoER utilizing three different substrates to synthesize the key Sacubitril precursor. The best performing variant, ClER M3 (M35C/Y76C/Q244G/V289P), enables substrate loadings of up to 100 mM with up to 99% yields and ee. This chemoenzymatic strategy provides a valuable platform for the reduction of challenging α,β-unsaturated carboxylic acids bearing large substituents, such as biphenyls or naphthyl motifs.
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