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Updated: Apr 20, 2026
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
Published on: March 21, 2018
High-sensitivity simultaneous discrimination and detection of structurally similar adenosine phosphates via DNA
Linna Li1, Yandi Hui1, Zhen Li1
1Key Laboratory of Synthetic and Natural Functional Molecular Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an, 710127, PR China.
Abstract:
Adenosine phosphates (ATP, ADP, and AMP) play critical roles in cellular processes, yet their simultaneous detection remains challenging due to high structural similarity. To overcome this limitation, we developed a nanopore-aptamer sensing platform capable of tracking single-molecule binding dynamics in real time. This method leverages the specific interactions between aptamers and each adenosine phosphate, enabling discrimination among ATP, ADP, and AMP at the single-molecule level. We demonstrate the high sensitivity and selectivity of this approach, which supports high-throughput analysis even in complex matrices. The platform was successfully applied to quantify ATP, ADP, and AMP in human serum, with excellent recovery rates (85.33%-97.53%) and precision (RSD < 7.03%), demonstrating its practical utility for multiplexed detection in biologically relevant environments. This work provides a powerful strategy for precise metabolite analysis and facilitates new studies on nucleotide-dependent pathways.
