Protocol for developing a surgically operated model to mimic human glioblastoma resection and recurrence

We present a protocol to develop a syngeneic surgical glioblastoma model using F98 cells in Fischer F344 rats. This model recapitulates tumor growth, surgical resection, and recurrence observed in patients. We describe procedures for F98 cell preparation, stereotactic implantation, MRI-guided tumor resection, and longitudinal MRI monitoring. This protocol provides a clinically relevant platform to investigate post-resection tumor recurrence and evaluate therapeutic strategies targeting residual disease.