Related Experiment Video
Updated: Apr 21, 2026
Development and Standardization of an Ex Vivo Micromethod for Intracellular Quantification of Vincristine in Primary ALL Cells by LC-MS/MS
Published on: January 23, 2026
Pilot study on differential gene expression in relation to vincristine response in canine transmissible venereal
Fabián D López-Valbuena1, William F Osorio-Zambrano2, André L Debiaso Rossi3
1Universidad Nacional de Colombia, Facultad de Medicina Veterinaria y de Zootecnia, Veterinary Pathology Research Group, Carrera 30 No. 45-03, Bogotá 111321, Colombia.
Background:
The canine transmissible venereal tumor (CTVT) is a naturally occurring clonal cancer that offers a unique model to study tumor evolution, immune evasion, and chemoresistance. Although vincristine induces complete remission in most cases, some tumors show partial response or resistance, and the molecular drivers of this variability remain unclear. While genomic and epigenetic studies have implicated multidrug resistance and immune modulation, transcriptional mechanisms underlying therapeutic outcomes are not fully characterized.
Objective:
To identify transcriptomic signatures and functional gene modules associated with vincristine response in CTVT showing complete response (CR), partial response (PR), or resistance (R).
Methods:
We analyzed RNA-Seq data from 10 CTVT tumors grouped by therapeutic response. After filtering and quality control, differential expression was assessed using DESeq2. Functional enrichment was evaluated using DAVID, and protein-protein interaction networks were built with STRING. Co-expression modules were identified via Pearson correlation and Louvain clustering.
Results:
We identified 97 DEGs (CR vs. R), 89 (PR vs. R), and 55 (CR vs. PR). Complete response tumors showed upregulation of immune-related (CD79A, CD19), extracellular matrix (ITGB3), and mitophagy genes (BCL2L13), while R tumors expressed markers of stress adaptation and resistance (GNG4, XPO1, KCNJ3). Partial response tumors displayed intermediate profiles (CSNK2B, DHX9). Protein-protein interaction networks showed limited connectivity, but co-expression modules revealed distinct expression patterns associated with response.
Conclusion:
This exploratory analysis characterizes transcriptional differences among response groups and identifies candidate genes and pathways that may contribute to vincristine sensitivity or resistance in CTVT. Considering the small number of resistant tumors included, these results are preliminary and require validation in larger, independent cohorts.
Related Concept Videos
01:03Rous Sarcoma Virus (RSV) and Cancer
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
01:03Rous Sarcoma Virus (RSV) and Cancer