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Identification of a Novel HLA-DPA1 Allele, HLA-DPA1*02:162, Using PolyseqOne and Oxford Nanopore Sequencing
Chaolan Liang1, Yan Yang1, Yafei Wu1
1Department of Blood Transfusion, Shenzhen Third People's Hospital, Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen, Guangdong Province, China.
HLA
|April 19, 2026
Summary
A novel Human Leukocyte Antigen (HLA) allele, HLA-DPA1*02:162, has been identified. It differs from a known allele by a single nucleotide substitution, impacting its genetic sequence.
Area of Science:
- Immunogenetics
- Molecular biology
- Human Leukocyte Antigen (HLA) system
Background:
- The Human Leukocyte Antigen (HLA) system plays a critical role in immune response and transplantation.
- Accurate HLA typing is essential for matching donors and recipients to prevent immune rejection.
- Novel HLA allele discovery contributes to a comprehensive understanding of HLA polymorphism.
Purpose of the Study:
- To characterize a newly identified HLA-DPA1 allele.
- To describe the genetic variation distinguishing this new allele from previously known ones.
Main Methods:
- Sequence analysis of the HLA-DPA1 gene.
- Comparison of nucleotide sequences between the novel and reference alleles.
Main Results:
- A novel HLA allele, designated HLA-DPA1*02:162, was identified.
- This allele differs from HLA-DPA1*02:02:02:01 by a single nucleotide substitution (c.509 C>T) at position 509 in exon 3.
- The substitution results in a missense mutation.
Conclusions:
- The discovery of HLA-DPA1*02:162 expands the known repertoire of HLA-DPA1 alleles.
- This finding highlights the ongoing need for high-resolution HLA typing to identify rare alleles.
- Understanding such variations is crucial for immunogenetic research and clinical applications.
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