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Updated: Apr 21, 2026

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

A Comprehensive Review on CRISPR-Based Screening and Its Applications.

Ali Saber Sichani¹, Mahmoud Hassani², Fatemeh Gila³

¹Department of Biology, Texas A&M University, College Station, TX, 77843, USA.

Molecular Biotechnology

|April 19, 2026

Summary

This summary is machine-generated.

CRISPR screens are powerful tools for studying gene function in cells. This review covers CRISPR library design, delivery, and selection methods for various applications like drug response and cancer research.

Keywords:

Arrayed screening CRISPR screening CRISPR/Cas9 High-throughput sequencing Pooled screening

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Area of Science:

Genomics
Molecular Biology
Biotechnology

Background:

CRISPR-based tools are now standard in biology and medicine.
CRISPR screens are essential for large-scale loss-of-function and perturbation studies.

Purpose of the Study:

To review the use of pooled CRISPR screens for interrogating gene function in living cells.
To contrast pooled and arrayed CRISPR screening formats.
To discuss recent applications and future directions of CRISPR screening.

Main Methods:

Focus on pooled CRISPR screens analyzing cell fitness or selectable markers.
Contrast with arrayed formats offering richer molecular readouts like transcriptome-wide changes.
Discuss library design, delivery, and selection strategies using various Cas nucleases (Cas9, Cas12, Cas13).

Main Results:

Different Cas nucleases expand the scope of genome and transcriptome perturbations that can be studied.
CRISPR screens have diverse applications in drug response, viral infection, and cancer biology.
Advancements in high-content technologies and data analysis are enhancing screening capabilities.

Conclusions:

CRISPR screening is a versatile technology for gene function analysis.
Improvements are driving the next generation of CRISPR-based screening studies.
Emerging diagnostic uses highlight the expanding role of CRISPR technology.