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Genome sequence of lytic phage phi1_164023 targeting ST23 KL1-type carbapenem-resistant Klebsiella pneumoniae

Huan Kuang1, Qingqing Fang1,2, Yu Feng3,4

  • 1Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China.

Microbiology Resource Announcements
|April 20, 2026
PubMed

Insights

We sequenced a novel phage genome from sewage, belonging to the Przondovirus genus. This phage effectively lyses carbapenem-resistant Klebsiella pneumoniae strains, offering potential for combating antibiotic resistance.

Area of Science:

  • Microbiology
  • Genomics
  • Bacteriophage research

Background:

  • Carbapenem-resistant Klebsiella pneumoniae (CRKP) poses a significant global health threat.
  • Bacteriophages are viruses that infect bacteria and are being explored as alternatives to antibiotics.
  • The Przondovirus genus is a group of bacteriophages with limited genomic characterization.

Purpose of the Study:

  • To characterize the genome of a novel phage with potential lytic activity against CRKP.
  • To provide genomic insights into the Przondovirus genus.

Main Methods:

  • Isolation of a bacteriophage from sewage.
  • Whole-genome sequencing and analysis of the phage.
  • Identification of protein-coding sequences and genomic features.

Main Results:

  • The complete genome of a Przondovirus phage was sequenced (39,059 bp, 53.11% GC content).
  • The genome encodes 55 protein-coding sequences and lacks tRNA genes.
  • The isolated phage demonstrated the capability to lyse ST23 KL1-type carbapenem-resistant Klebsiella pneumoniae strains.

Conclusions:

  • The characterized phage represents a potential candidate for phage therapy against CRKP infections.
  • Genomic data contributes to the understanding of the Przondovirus genus and phage-host interactions.
Keywords:
Klebsiella pneumoniaecarbapenem resistancehypervirulentphagephage therapy

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