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Correlation of D-dimer Measurement Values Using Quantum Dots Fluorescence Immunochromatographic Assay and
Alif Ainudin1, Ferdy Royland Marpaung2,3, Paulus Budiono Notopuro2,3
1Clinical Pathology Specialization Programme, Faculty of Medicine - Universitas Airlangga, Surabaya, Indonesia.
Introduction:
D-dimer, a fibrin degradation product, is widely used in the diagnosis of thrombotic disorders and in clinical decision-making. Quantum dots (QDs) fluorescence immunochromatography has emerged as a rapid method for detecting protein biomarkers; however, comparative data with the established latex-enhanced immunoturbidimetric assay remain limited.
Methods:
A laboratory-based method comparison study was conducted using 80 consecutively collected plasma samples from patients at Dr. Soetomo General Academic Hospital, Surabaya. D-dimer levels were measured using a QDs fluorescence immunochromatography assay (QD-S2000, Vazyme, China) and a latex-enhanced immunoturbidimetric assay (Sysmex CS-2500, Sysmex Corporation, Japan).Correlation and agreement between methods were assessed using Spearman correlation analysis and Bland-Altman plots.
Results:
Among the 80 samples (60% female, 40% male), a very strong correlation was observed between the two methods (Spearman r = 0.951, p < 0.001). Bland-Altman analysis demonstrated good agreement, with most data points falling within the 95% limits of agreement. Although the QDs fluorescence immunochromatography method consistently produced higher absolute D-dimer values, both assays showed comparable trends across the measurement range.
Conclusion:
QDs fluorescence immunochromatography and the latex-enhanced immunoturbidimetric assay demonstrated strong correlation and acceptable agreement in D-dimer measurement. Despite yielding higher absolute values, the QDs-based method showed consistent performance, supporting its potential as a reliable and rapid alternative for clinical D-dimer assessment.
