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A Live-Cell Analytical Platform by Using Strigolactones-Induced Dimerization System.

Kun Zang1, Xinwei Lu1, Chaoliang Diao1

  • 1State Key Laboratory of Synergistic Chem-Bio Synthesis, School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.

Analytical Chemistry
|April 20, 2026
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Summary
This summary is machine-generated.

A novel chemical-induced dimerization (CID) system using strigolactones (SLs) enables precise control of cellular processes. This robust SLs-CID platform allows for live-cell analysis and sensing of intracellular activities with high specificity.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biotechnology

Background:

  • Chemical-induced dimerization (CID) is crucial for live-cell analysis but often suffers from poor orthogonality and complex behaviors.
  • Existing CID systems face limitations in specificity, concentration dependence, and interaction with endogenous pathways, hindering intracellular applications.

Purpose of the Study:

  • To develop a novel, highly specific, and orthogonal CID system based on the strigolactones (SLs) signaling pathway for live-cell analysis.
  • To engineer a versatile SLs-induced dimerization (SLs-CID) platform for precise intracellular perturbations and sensing.
  • To demonstrate the application of SLs-CID in monitoring Caspase-3 activity and constructing an intracellular logic gate.

Main Methods:

  • Utilized strigolactones analog GR24 to induce dimerization between DAD2 and MAX2514.
  • Systematically evaluated SLs-CID system performance, including specificity, orthogonality, reversibility, cytotoxicity, and kinetics.
  • Integrated SLs-CID with single-molecule fluorescence triple-correlation spectroscopy (FTCS) to create an intracellular 'AND' logic gate.

Main Results:

  • The developed SLs-CID system demonstrated high specificity, orthogonality, reversibility, minimal cytotoxicity, and saturable kinetics.
  • Successfully implemented in situ monitoring of Caspase-3 activity in single living cells by precisely modulating protein distribution.
  • Constructed a functional intracellular 'AND' logic gate using SLs-CID and FTCS, achieving high-specificity analytical readout.

Conclusions:

  • The SLs-induced dimerization system offers a versatile and robust tool for controlled intracellular perturbations and sensing.
  • This chemically programmable framework enables advanced live-cell analysis and the development of sophisticated cellular logic gates.
  • The SLs-CID platform significantly advances the field of intracellular analysis and biosensing.