Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Life cycle, seasonal- and tissue-dependent metabolite composition, and high (E)-Ligustilide content characterize the ethnobotany of a Native American medicinal plant, Lomatium foeniculaceum.

Journal of natural medicines·2026
Same author

Protodesilylation as a Stereochemical Switch: Divergent Asymmetric Synthesis from Chiral Skipped Dienes.

Organic letters·2026
Same author

CD4/CD8 ratio is associated with structural reorganization of vaccine-induced immune responses in people living with HIV.

Frontiers in immunology·2026
Same author

Trichodysplasia Spinulosa in a Japanese Pediatric Patient.

The Journal of dermatology·2026
Same author

Convexal subarachnoid hemorrhage due to infective endocarditis: a rare forensic autopsy case.

Journal of forensic and legal medicine·2026
Same author

An imported case of human granulocytic anaplasmosis presenting with altered mental status: A case report.

IDCases·2026

Related Experiment Video

Updated: Apr 22, 2026

Author Spotlight: Enhancing Understanding and Treatment Strategies with the NEC-on-a-Chip Model
06:51

Author Spotlight: Enhancing Understanding and Treatment Strategies with the NEC-on-a-Chip Model

Published on: July 28, 2023

2.0K

Modeling human enterovirus A71 infection using an intestinal microphysiological system.

Hiroki Futatsusako1,2, Sayaka Deguchi1, Kaori Kosugi1

  • 1Department of Synthetic Human Body System, Medical Research Laboratory, Institute of Integrated Research, Institute of Science Tokyo, Tokyo, Japan.

Journal of Virology
|April 21, 2026
PubMed
Summary

Enterovirus A71 (EV-A71) infects the intestine without causing damage, but doesn't trigger interferon (IFN) release. Interferon treatment restricted viral replication, highlighting its importance in controlling EV-A71 infection.

Keywords:
embryonic stem cellsgut-on-a-chiphand, foot, and mouth diseaseorgan-on-a-chippluripotent stem cell

More Related Videos

Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality
10:56

Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality

Published on: May 5, 2022

25.4K
Author Spotlight: Developing Immunocompetent Organ-on-Chip Models for Infectious Disease Research
08:48

Author Spotlight: Developing Immunocompetent Organ-on-Chip Models for Infectious Disease Research

Published on: May 24, 2024

2.7K

Related Experiment Videos

Last Updated: Apr 22, 2026

Author Spotlight: Enhancing Understanding and Treatment Strategies with the NEC-on-a-Chip Model
06:51

Author Spotlight: Enhancing Understanding and Treatment Strategies with the NEC-on-a-Chip Model

Published on: July 28, 2023

2.0K
Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality
10:56

Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality

Published on: May 5, 2022

25.4K
Author Spotlight: Developing Immunocompetent Organ-on-Chip Models for Infectious Disease Research
08:48

Author Spotlight: Developing Immunocompetent Organ-on-Chip Models for Infectious Disease Research

Published on: May 24, 2024

2.7K

Area of Science:

  • Virology
  • Gastroenterology
  • Immunology

Background:

  • Enterovirus A71 (EV-A71) causes hand, foot, and mouth disease, with intestinal replication preceding potential neurological complications.
  • Understanding intestinal pathophysiology during EV-A71 infection is crucial for preventing severe outcomes.
  • Current knowledge of EV-A71's intestinal effects is limited.

Purpose of the Study:

  • To investigate the intestinal response to EV-A71 infection using a human pluripotent stem cell-derived intestinal microphysiological system (MPS).
  • To analyze viral replication, host cell response, and the role of interferons (IFNs) in the intestinal environment during EV-A71 infection.

Main Methods:

  • Development of an intestinal MPS using human pluripotent stem cells and microfluidic devices.
  • Infection of the intestinal MPS with EV-A71 and monitoring of viral titers.
  • Assessment of morphological changes, epithelial cell marker expression, and interferon secretion.
  • Treatment with recombinant interferons to evaluate their antiviral effects.

Main Results:

  • EV-A71 was detectable in the intestinal MPS supernatant for 14 days post-infection.
  • No significant morphological changes or alterations in epithelial markers were observed, indicating persistent infection without overt damage.
  • Viral infection did not increase endogenous interferon secretion.
  • Recombinant IFN treatment reduced viral mRNA levels and increased innate immune gene expression.

Conclusions:

  • The intestinal MPS supports persistent EV-A71 infection without causing significant epithelial damage.
  • Interferon signaling plays a critical role in restricting EV-A71 replication within the intestinal environment.
  • The intestinal MPS is a valuable platform for studying EV-A71 pathogenesis and evaluating antiviral therapies.