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Updated: Apr 23, 2026

Extraction and Analysis of Cortisol from Human and Monkey Hair
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Testing for hydroxyzine in human hair after a single controlled administration: First quantitative data.

Nadia Arbouche1, Laurie Gheddar1, Jean-Sébastien Raul1

  • 1Institut de Médecine Légale, Strasbourg, France; and.

Therapeutic Drug Monitoring
|April 21, 2026
PubMed
Summary
This summary is machine-generated.

This study measured hydroxyzine in human hair after a single dose, finding measurable levels and hair color influence. This supports hair analysis for drug-facilitated crime investigations.

Keywords:
LC-MS/MShair analysishydroxyzinesingle administration

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Area of Science:

  • Forensic Toxicology
  • Pharmacology
  • Analytical Chemistry

Background:

  • Hydroxyzine, a first-generation antihistamine, is used for anxiety and sedation.
  • It has been implicated in drug-facilitated crimes.
  • Limited data exists on hydroxyzine in hair after single use, hindering forensic interpretation.

Purpose of the Study:

  • To provide the first quantitative data on hydroxyzine concentrations in human hair.
  • To assess hydroxyzine incorporation after a single oral dose.
  • To evaluate the utility of hair analysis in forensic investigations.

Main Methods:

  • Three healthy volunteers received a single 25-mg oral dose of hydroxyzine.
  • Hair samples (proximal 0-2 cm) were collected one month post-administration.
  • Quantification used a validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method.

Main Results:

  • Hydroxyzine was detected in all hair samples, ranging from 15 to 63 pg/mg.
  • Higher concentrations were observed in subjects with dark hair compared to blond hair.
  • Cetirizine, a metabolite, was also detected at 7-23 pg/mg.

Conclusions:

  • This study establishes reference data for hydroxyzine in hair after a single dose.
  • Hydroxyzine incorporation into hair is influenced by hair pigmentation.
  • Hair analysis is a valuable complementary tool for retrospective investigations, including drug-facilitated crimes.