DNA Helix Bundle-Encoded Multi-Bit Information Readout by Sapphire-Supported Nanopores

Area of Science:

• Biotechnology
• Nanotechnology
• Data Storage

Background:

• The demand for high-capacity data storage fuels innovation in memory technologies.
• DNA offers a promising medium for high-density data storage due to its molecular nature.
• Nanostructured DNA combined with nanopore detection presents a viable storage solution.

Purpose of the Study:

• To demonstrate a flexibly programmable and secure DNA data storage readout scheme.
• To utilize sapphire-supported, low-noise nanopores for detecting nanostructured DNA.
• To achieve a high signal-to-noise ratio for reliable data retrieval.

Main Methods:

• Employing four-helix bundle (4HB) DNA nanostructures for data encoding.
• Utilizing sapphire-supported nanopores for low-noise DNA detection.
• Applying decision-tree supervised learning for message classification.

Main Results:

• Achieved a high signal-to-noise ratio of approximately 22 with 250 kHz filtering.
• Successfully classified up to ~93% of encoded messages using machine learning.
• Demonstrated the feasibility of encoding letters and messages for information retrieval.

Conclusions:

• Sapphire-supported nanopores provide a robust platform for DNA data storage.
• The proposed scheme offers a secure and programmable method for data readout.
• This technology shows significant potential for future high-density information storage solutions.