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Metagenomic Analysis of Silage
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Rumen Mycobiome Thiamine Metabolism Contributes to Subacute Rumen Acidosis Tolerance in Goats Through Enhancing
Jingyi Xu1,2, Xiaodong Chen1,2,3, Yi Ma4,5
1College of Animal Science and Technology Northwest A&F University Yangling Shaanxi China.
Exploration (Beijing, China)
|April 22, 2026
Summary
Rumen fungi, particularly Aspergillus bombycis, enhance thiamine metabolism to promote rumen epithelial cell proliferation and improve tolerance to subacute rumen acidosis (SARA) in dairy goats.
Area of Science:
- Rumen microbiology
- Animal metabolism
- Molecular biology
Background:
- Subacute rumen acidosis (SARA) is a significant metabolic disorder affecting dairy ruminants and milk production.
- The precise mechanisms by which rumen fungi impact SARA susceptibility and tolerance are not fully understood.
- Investigating rumen fungal functions and their interaction with host physiology is crucial for understanding SARA.
Purpose of the Study:
- To explore the relationship between rumen fungal metabolic activities and host SARA tolerance.
- To examine rumen epithelial miRNA-mRNA correlations and cell subtype variations influenced by fungi.
- To elucidate the molecular mechanisms underlying SARA tolerance mediated by rumen fungi.
Main Methods:
- Identification of SARA-susceptible and SARA-tolerant goats based on rumen pH monitoring during high-concentrate feeding.
- Analysis of rumen fungal abundance and metabolic functions (cellulose, amino acid, thiamine).
- Investigation of miRNA-mRNA correlations, focusing on the Aspergillus bombycis-thiamine-IGFBP2 axis and downstream signaling pathways (ERK, PI3K/AKT).
Main Results:
- SARA-susceptible goats showed reduced anaerobic fungi and altered metabolic functions, while Aspergillus bombycis enrichment was linked to SARA tolerance.
- Aspergillus bombycis, via thiamine metabolism, downregulates chi-miR-17-26080, increasing IGFBP2 expression.
- Thiamine supplementation upregulated IGFBP2, activating IGF1 signaling and promoting rumen epithelial cell proliferation (CDK4, Cyclin A2), enhancing volatile fatty acid absorption.
Conclusions:
- Rumen fungi, particularly Aspergillus bombycis, play a key role in SARA tolerance through thiamine production.
- The IGFBP2/IGF1 signaling pathway activated by thiamine promotes rumen epithelial cell proliferation and enhances volatile fatty acid absorption.
- These findings offer insights into preventing SARA in ruminants by modulating rumen fungal communities and thiamine metabolism.
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