Related Experiment Video
Updated: Apr 24, 2026

Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography TLC Coupled with Gas-Liquid Chromatography GLC
Published on: March 18, 2011
TgSEC14-LTP1 is a vacuole-secreted SEC14/CRAL-TRIO-like protein required for host DAG flux and PC homeostasis in T.
Rosalba Cruz-Miron1, Christopher Swale2, Qinli Liang1
1Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, CA, USA.
Abstract:
Intracellular apicomplexan parasites depend on host-derived lipids to expand the parasitophorous vacuole (PV) and replicate. Toxoplasma gondii secretes numerous dense granule proteins (GRAs) for PV remodeling, but PV lumen factors that selectively bind host lipids and shape host-to-parasite lipid flux remain poorly defined. Here, we identify TgSEC14-LTP1, a previously uncharacterized GRA with a divergent SEC14/CRAL-TRIO domain. Structural and genetic analyses show that TgSEC14-LTP1 is secreted into the PV lumen and is essential for parasite replication. Lipidomic profiling, protein-lipid pulldown assays, 13C-labeling-based lipid flux analysis, and fluorescent lipid uptake assays show that TgSEC14-LTP1 binds phosphatidylcholine (PC) and diacylglycerol (DAG) and is required for efficient host-to-parasite PC and DAG flux and parasite lipid homeostasis. TgSEC14-LTP1 depletion reduces parasite PC and DAG pools, disrupts bulk PC synthesis and parasite biogenesis, delays egress, and ultimately impairs growth. These findings identify TgSEC14-LTP1 as a key Sec14-like PV lumen factor that links lipid binding to host lipid acquisition and PC homeostasis, underscoring the importance of host lipid scavenging for parasite survival.
More Related Videos
10:23Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
Published on: April 17, 2017
12:15The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analy
Published on: October 3, 2017
Related Concept Videos
Overview of Secretory Vesicles
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Clathrin Coated Vesicles
Coat Assembly and GTPases
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
IP3/DAG Signaling Pathway
Protein Translocation Machinery on the ER Membrane
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
Tail-anchoring of Proteins in the ER Membrane