Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview of Microscopy Techniques

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comparison of alpha-particle track characteristics in GAGG and biological tissues for microdosimetry using experimentally validated GATE simulations with high-resolution track imaging.

Physics in medicine and biology·2026

Same author

Investigation of post-irradiation molecular changes in LiPCDA-based radiochromic film (Gafchromic EBT4) using Raman spectroscopy.

Biomedical physics & engineering express·2026

Same author

Development of Optical-Guiding Scintillators with Ultrafine (~12 μm) Uniform Scintillator Cores for High-Resolution X-Ray Imaging.

Materials (Basel, Switzerland)·2026

Same author

Investigation on the mechanism underlying UV-induced reductions in radiophotoluminescence and electron spin resonance signals in Ag-doped alkali phosphate glass.

International journal of radiation biology·2026

Same author

Investigation of the Microstructure and Scintillation Properties of Ce-Doped CaF<sub>2</sub>/LiF Eutectics for Thermal Neutron Detection.

Materials (Basel, Switzerland)·2026

Same author

Performance evaluation of a flexible GAGG sheet for surface imaging of x-rays from computed tomography (CT) and linear accelerator (LINAC).

Biomedical physics & engineering express·2026

Same journal

Effective contrast-enhanced preprocessing for intracranial artery segmentation in digital subtraction angiography.

Physics in medicine and biology·2026

Same journal

Improving Plan Quality in Adaptive Proton Therapy Using an Interactive Dose Modification Tool.

Physics in medicine and biology·2026

Same journal

Technical Note: Real-Time MLC Control and Latency Measurement Optimization with External Verification.

Physics in medicine and biology·2026

Same journal

Fetus-Specific Hematopoietic Stem Cell Dosimetry Framework for Leukemia-Relevant Target Cells During Prenatal Development.

Physics in medicine and biology·2026

Same journal

Deep learning-based dose prediction to enhance planning efficiency in cervical brachytherapy with hybrid applicators.

Physics in medicine and biology·2026

Same journal

Corrigendum: Referenceless MR thermometry-a comparison of five methods (2017<i>Phys. Med. Biol</i>.<b>62</b>1-16).

Physics in medicine and biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 24, 2026

$Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2$

Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2

Published on: December 8, 2016

Proton microbeam characterization using a single-particle scintillator.

Katsunori Yogo¹, Ryu Okada¹, Tatsuya Kameyama²

¹Graduate School of Medicine, Nagoya University, 1-1-20 Daiko-minami, Higashi-ku, Nagoya, Aichi 461-8673, Japan.

Physics in Medicine and Biology

|April 22, 2026

Summary

This summary is machine-generated.

This study introduces a simple method to measure proton microbeam dose and width using a single scintillator particle. The technique achieves sub-micron resolution, enhancing precision in radiobiology research.

Keywords:

dosimetry micro-scintillator proton microbeam

More Related Videos

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Published on: February 3, 2018

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

Related Experiment Videos

Last Updated: Apr 24, 2026

$Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2$

Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2

Published on: December 8, 2016

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Published on: February 3, 2018

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

Area of Science:

Physics
Materials Science
Biotechnology

Background:

Accurate characterization of proton microbeams is crucial for precise radiobiological studies.
Existing methods for microdosimetry can be complex and lack high spatial resolution.

Purpose of the Study:

To develop a simple, high-resolution method for measuring local dose and lateral beam width of proton microbeams.
To utilize light emission from a single scintillator particle for dosimetry and beam profiling.

Main Methods:

Employed silver-activated zinc sulfide (ZnS:Ag) scintillator powder for its dose response and emission properties.
Used a 3.4 MeV proton microbeam delivered by the Single-Particle Irradiation system to Cell (SPICE).
Recorded scintillator particle emission via a microscope-coupled camera and scanned the beam to measure intensity variations.

Main Results:

Luminescence intensity showed a linear correlation with irradiation time and beam position.
Achieved precise beam center localization despite particle diameter exceeding beam width.
Measured lateral beam widths of 1.9 ± 0.3 μm (X) and 1.8 ± 0.4 μm (Y) with sub-micron resolution.

Conclusions:

The developed method allows simultaneous dose and beam width estimation from a single scintillator particle.
Offers a practical, accurate tool for microbeam characterization, improving dosimetry and targeting in radiobiology.
Enables beam profile reconstruction with spatial precision exceeding optical pixel resolution.