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Updated: Apr 24, 2026

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis
Published on: June 13, 2021
Clathrin adaptor EPSIN1 (EPS1) modulates plasma membrane abundance of PLEIOTROPIC DRUG RESISTANCE PDR9 for effective
Nga T Nguyen1, Erica D LaMontagne-Mueller1, Gabriela F Kauffman1,2
1Division of Biochemistry, Interdisciplinary Plant Group (IPG), University of Missouri, Columbia, MO, 65211, USA.
Abstract:
The cellular machinery that directs the indole-3-butyric acid (IBA) effluxer PLEIOTROPIC DRUG RESISTANCE 9 (PDR9) to the outer plasma membrane (PM) domain of the root epidermis remains poorly understood. This study combines biochemical fractionation, live-cell imaging, and physiological assays to identify the trans-Golgi Network-localized clathrin adaptor EPSIN1 (EPS1) as a novel modulator of PDR9 abundance at the root PM for IBA-dependent physiological processes. These findings expand EPS1's roles from plant immunity to hormone homeostasis, thereby advancing the limited understanding of biological functions for plant EPSINs.
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