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Related Concept Videos

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The energy released from the breakdown of the chemical bonds within nutrients can be stored either through the reduction of electron carriers or in the bonds of adenosine triphosphate (ATP). In living systems, a small class of compounds functions as mobile electron carriers, molecules that bind to and shuttle high-energy electrons between compounds in pathways. The principal electron carriers that will be considered originate from the B vitamin group and are derivatives of nucleotides; they are...
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Muscle fatigue refers to the decline in a muscle's ability to maintain the force of contraction after prolonged activity. It primarily stems from changes within muscle fibers. Even before experiencing muscle fatigue, one may feel tired and have the urge to stop the activity. This response, known as central fatigue, occurs due to changes in the central nervous system, namely the brain and spinal cord. While there is no single mechanism that induces fatigue, it may serve as a protective...
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Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
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Related Experiment Video

Updated: Apr 24, 2026

Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue
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D- and L-lactate dehydrogenase reactions increase pH to lactate production and decrease pH to pyruvate production.

Robert Robergs1,2,3, Marek Nalos3,4, Petr Kelbich5

  • 1School of Exercise and Nutrition Sciences, Faculty of Health, Queensland University of Technology, Kelvin Grove, QLD, Australia.

Frontiers in Molecular Biosciences
|April 23, 2026
PubMed
Summary
This summary is machine-generated.

The lactate dehydrogenase (LDH) reaction consumes or releases hydrogen ions (H+), altering pH. Pyruvate reduction to lactate increases pH, while lactate oxidation to pyruvate decreases pH, impacting metabolic acidosis understanding.

Keywords:
acidosisalkalosisenzyme catalysismetabolismspectrophotometry

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Area of Science:

  • Biochemistry
  • Physiology

Background:

  • Metabolic acidosis in extrahepatic tissues is traditionally attributed to increased lactic acid production.
  • The role of the lactate dehydrogenase (LDH) reaction in pH changes requires further clarification.

Purpose of the Study:

  • To investigate the impact of the LDH reaction on pH by measuring pH changes during lactate and pyruvate production.
  • To determine if the LDH reaction directly produces acids or anions.

Main Methods:

  • In vitro experiments were conducted using D- and L-LDH reactions with varying substrate concentrations.
  • pH changes were continuously monitored using a glass polymer pH electrode after LDH addition.
  • Product concentrations (lactate and pyruvate) were measured intermittently.

Main Results:

  • Pyruvate reduction to lactate consumed H+, increasing pH (6.3-10.5).
  • Lactate oxidation to pyruvate released H+, decreasing pH (8.8-6.9).
  • Product concentrations were generally low, with pyruvate production yielding the lowest levels.

Conclusions:

  • The LDH reaction directly influences pH through hydrogen ion consumption or release.
  • These findings provide a basis for understanding LDH's role in biological pH regulation and metabolic acidosis.