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Aptamer-Based Approaches for Influenza Virus Detection: A Systematic Review
Javad Charostad1, Zohreh-Al-Sadat Ghoreshi2, Nasir Arefinia2
1Department of Microbiology, Faculty of Medicine Shahid Sadoughi University of Medical Sciences Yazd Iran.
Health Science Reports
|April 23, 2026
Summary
Aptamer-based tools show promise for accurate influenza virus detection, offering a faster and more stable alternative to traditional methods. These advanced diagnostics are crucial for improving global public health surveillance.
Area of Science:
- Biotechnology
- Molecular Diagnostics
- Biosensors
Background:
- Influenza virus poses a significant global health challenge due to diagnostic limitations.
- Traditional methods for influenza detection often lack speed and accuracy.
- Aptamer-based technologies offer a novel approach to overcome these diagnostic hurdles.
Purpose of the Study:
- To systematically review aptamer-based tools for influenza virus detection.
- To evaluate the efficacy and limitations of these novel diagnostic methods.
- To identify areas for future research and development in aptamer diagnostics for influenza.
Main Methods:
- Systematic review following PRISMA guidelines.
- Searched multiple databases (PubMed, Web of Science, Scopus, Embase, Google Scholar) and grey literature.
- Included 22 studies from 1432 identified articles, focusing on aptamers and influenza detection.
Main Results:
- Diverse aptamer techniques identified, including FRET, electrochemical, and SERS-based aptasensors.
- Systematic Evolution of Ligands by Exponential Enrichment (SELEX) is the primary aptamer development method.
- FRET methods achieved detection limits of 0.43 ng/mL; electrochemical methods showed picomolar sensitivity.
Conclusions:
- Aptamer-based diagnostics present a cost-effective, stable, and rapid alternative for influenza detection.
- These tools are suitable for rapid, field-deployable applications and multiplex detection.
- Further optimization is needed for sensitivity and technological simplicity for widespread clinical use.
